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Restriction Map Construction

Restriction Map Construction. Restriction Enzyme Analysis: (Double Digestion) Cell Bio Lab. Gel Data U = 17 kb E = 1, 3, 7 kb H = 4, 13 kb E + H = 1, 3, 6 kb. 3E. 7E. 3E. 3E. 1E. E. E. E. E. E. E. E. E. 7. 7. 3. 3. 3. 3. 3. 3. 1. 1. 4H. 13H. H. 4. 13. E. H.

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Restriction Map Construction

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  1. Restriction Map Construction Restriction Enzyme Analysis: (Double Digestion) Cell Bio Lab

  2. Gel Data U = 17 kb E = 1, 3, 7 kb H = 4, 13 kb E + H = 1, 3, 6 kb

  3. 3E 7E 3E 3E 1E E E E E E E E E 7 7 3 3 3 3 3 3 1 1 4H 13H H 4 13 E H E E E 6 3 1 3 3 1 Gel Data U = 17 kb E = 1, 3, 7 kb (3, 3) H = 4, 13 kb E + H = 1, 3, 6 kb 3E1H6E3E3E1 = 1E3E3E6H1E3

  4. E E E E E E E E E E 7 7 3 3 3 3 1 1 1 1 3E 7E 3E 1E 1E 1E 1E 4H 13H H 4 13 E E H E E E E 6 3 1 3 1 1 1 1 Gel Data U = 17 kb E = 1, 3, 7 kb (3, 1,1,1) H = 4, 13 kb E + H = 1, 3, 6 kb 3E1H6E3E1E1E1E1 = 1E1E1E1E3E6H1E3

  5. More Restriction Maps that fit the above Gel data are waiting for you to discover. • Have fun!!!!!

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