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Lipid-dependent surface transport of the proton pumping

Lipid-dependent surface transport of the proton pumping. ATPase: A model to study plasma membrane biogenesis in yeast. Fan Mengdi Li Yandi. The Points. Introduction Biogenesis and transport of the proton pumping H + -ATPase. The general introduction of the H+-ATPase in yeast.

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Lipid-dependent surface transport of the proton pumping

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  1. Lipid-dependent surface transport of the proton pumping ATPase: A model to study plasma membrane biogenesis in yeast Fan Mengdi Li Yandi

  2. The Points • Introduction • Biogenesis and transport of the proton pumping H+-ATPase

  3. The general introduction of the H+-ATPase in yeast A good model to study plasma membrane biogenesis

  4. The general introduction • The function of ATPase : • maintenance of the electrochemical gradient across the membrane • regulating intracellular PH • symporters

  5. How did the H+-ATPase transport to the plasma membrane? Begin: oligomerization and package Surface transport of the ATPase Raft association Stable integrate at the plasma membrane protein lipid

  6. How did the H+-ATPase transport to the plasma membrane? • Every step needs the help of these lipids: sterols sphingolipids

  7. Step 1: oligomerization C26 protein Protein-lipid complex Pma1 The ER lipid In the ER, Pma1form a homo-oligomeric complex—the protein-lipid complex

  8. Step 2: package Protein-lipid complex The ER coat protein complex II(a kind of transport vesicle) The protein-lipid complex then packaged into a larger subclass of COPII

  9. Step 3: modify of the Pma1 Protein-lipid complex P- Golgi body coat protein complex II The Pma1 becomes phosphorylated on multiple serine and threonine residues

  10. Step 4: Pma1 transport to the cell surface Protein-lipid complex Golgi body C26 coat protein complex II The lipid raft Pma1 is transported to the cell surface

  11. How did the H+-ATPase transport to the plasma membrane? • Every step needs the help of these lipids: sterols sphingolipids

  12. Step 5: Stable integrate at the plasma membrane H+ C26 ATP Lipid raft association

  13. Two mutant of Pma1 Affect the stability, targeting, and detergent solubility during its transport to the surface

  14. The first mutant:Pmal-7 Vacuole Protein-lipid complex Golgi body coat protein complex II Pmal-7:mistargeted from the Golgi to the vacuole without reaching the cell surface.

  15. The second mutant:Pmal-10 C26 Pmal-10: fails to become stabilized on the membrane, but to delivered to the vacuole, then degradation. vacuole

  16. Two mutant of Pma1 . The mutant indicate: lipid raft association may play important roles in maintaining protein stability at the plasma membrane.

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