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A. CCAAT---N9----CCACG GGTTA---N9----GGTGC. ERSE consensus. mCRELD2-ALG12 ERSE. -254. -236. CCAAT TGAGGCTCG CCACG. mCRELD2-ALG12 ERSE m1. CCAAT TGAGGCTCG gatgt. mCRELD2-ALG12 ERSE m2. gacta TGAGGCTCG CCACG. Relative luciferase activity. B. Tg. 0. 50. 100. 150. 200. 250. -.
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A CCAAT---N9----CCACG GGTTA---N9----GGTGC ERSE consensus mCRELD2-ALG12 ERSE -254 -236 CCAATTGAGGCTCGCCACG mCRELD2-ALG12 ERSE m1 CCAATTGAGGCTCGgatgt mCRELD2-ALG12 ERSE m2 gactaTGAGGCTCGCCACG Relative luciferase activity B Tg 0 50 100 150 200 250 - pGL3b + ALG12 ERSE - -361/+11 + - -361+11 m1 + - -361/+11m2 + Relative luciferase activity C Tg 0 10 20 30 40 - pGL3b + CRELD2 - -229/-396 + - -229/-396 m1 + - -229/-396 m2 +