1 / 11

Nanopore Membranes for Single-Molecule Detection and Characterization

Nanopore Membranes for Single-Molecule Detection and Characterization. Anna Schibel, Ryuji Kawano and Henry S. White University of Utah October 26, 2007. The Glass Nanopore (GNP) Membrane . Zhang et al. Anal. Chem. 2007 , 79 , 4778-4787. . Fabrication of the GNP Membrane. Bo Zhang.

paul
Download Presentation

Nanopore Membranes for Single-Molecule Detection and Characterization

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Nanopore Membranes for Single-Molecule Detection and Characterization Anna Schibel, Ryuji Kawano and Henry S. White University of Utah October 26, 2007

  2. The Glass Nanopore (GNP) Membrane Zhang et al. Anal. Chem. 2007, 79, 4778-4787.

  3. Fabrication of the GNP Membrane Bo Zhang Nanopore membrane Nanopore electrode Nanodisk electrode Zhang, B.; Galusha, J.; Wang, G.; Bergren, A. J.; Jones, R. M.; White, R. J.; Ervin, E. N.; Cauley, C. C.; Shiozawa, P.; White, H. S. Anal. Chem. 2007, 79, 4778-4787.

  4. Stochastic Single-Molecule Detection HL insertion Single Binding Events Applied Voltage -40 mV 250 nm pore 1 M KCl 10 mM Potassium phosphate buffer pH 7.4 3 M aHL backside 50 M s7CD frontside Unbound State Bound State - -Hemolysin (HL) Gu et al., Science 2001, 291, 636-640 - hepta-sulfano--cyclodextrin (s7CD) R = -OSO3-

  5. Stochastic Single-Molecule Detection i 1 s a7CD Electrostatic trapping Clement Osei Akoto Rainer Group R = NH3+ GNP radius - 95 nm Voltage in wrt cis side 50 M a7CD detection cis - Reference Electrostatically trap molecule inside HL channel trans + - a7CD + + + 50 M a7CD

  6. Nanoparticle Detection Gold Nanoparticle with Tiopronin Monolayer 2nm Wang, G., Single Nanoparticle Sensing with Protein Ion Channel Embedded in Lipid Bilayer Supported By Glass Nanopore Membrane (unpublished)

  7. Characterization of Hairpin DNA Structures Common structure 9bp-GC 9bp-AT 9bp-TG

  8. Discrimination Of Individual DNA Hairpin Molecules 9 bp-TG 9 bp-AT 1 sec 1 sec 1 sec 9 bp-GC Current ~1 minute

  9. Heparin Detection - - - - - Heparin

  10. Applications In Development • Biomedical Analysis • DNA sequencing • Heparin analysis • Homeland Defense • Chemical warfare agent detection • Environmental Analysis • Ultra-trace level detection of pollutants • Nano-particle analysis

More Related