Introduction Membrane Permeation System Experimental Section

IntroductionMembrane Permeation SystemExperimental Section Presented By: Rich Dominiak Laura Kuczynski John Roszko

Introduction • Mass transfer through various membranes is receiving increased attention • Drug delivery through polymer membranes and human or animal skin has become a challenging research area • In vitro setups are used to make permeation measurements for membrane-moderated controlled release of drugs • The effect of the diffusion boundary layer on permeation has not been studied

Introduction • Permeation rate data can be easily distorted by the diffusion boundary layers on the membrane • The rate of drug permeation should be measured using a calibrated permeation cell • In this study • The hydrodynamic characteristics of the an in-vitro membrane permeation cell developed by Chien and Valia were investigated • Determine if it can be calibrated as a standardized in-vitro system

Membrane Permeation System • Membrane surface area – 0.64 cm2 • Star head magnetic stirrer • Donor and receptor reservoirs • 3.5 mL capacity • Completley enclosed • Jacketed for temperature control

Experimental Section • Objective: To obtain the aqueous solubilities of benzoic acid in various PEG 400 solutions • Saturated solutions of benzoic acid in water with 0-40% PEG 400 were prepared by placing an excess of benzoic acid in test tubes with the PEG solutions • They were placed in a shaking water bath for 24 hours

Experimental Section • Filtered through 0.45 um Millipore filter • Diluted with solution medium that was used in the test tubes • Concentrations were analyzed using a UV/vis spectrophotometer • Viscosity was measured using and Ostwald capillary viscometer

Experimental Section • Diffusion coefficients were calculated from literature values based on the diffusivity in pure water at 25 C • Wilke equation – 1.082 x 10-5 cm2/s • Thin benzoic acid disks were made by pouring fused benzoic acid into a metal mold positioned on a pill tile • Mounted to permeation cell

Experimental Section • 3.5 mL of each solution (previously made) were pipetted into the receptor compartment while the donor compartment remained empty • At time intervals, 20 uL were withdrawn and diluted to 10 mL samples • These were analyzed with the UV spectrophotometer

Introduction Membrane Permeation System Experimental Section

Introduction Membrane Permeation System Experimental Section

Presentation Transcript

Experimental Design: Introduction

Theoretical and Experimental Studies on Electroosmotic Membrane Nanopumps

Introduction to Experimental Design

Experimental Designs (Introduction)

Introduction to Experimental Economics

Introduction to experimental design

Introduction Section

Introduction and Experimental

Introduction to Experimental Design

Membrane permeation

Introduction to Experimental Design

Introduction to Experimental Research

WWT experimental automation system

Outline Introduction Experimental Overview Inclusive Cross Section Neutral Pions in Jets

Membrane permeation is achieved by a variety of mechanisms

APSTAT Section 3 Experimental Design

INTRODUCTION TO THE CELL MEMBRANE

DRUG PERMEATION THROUGH SKIN

Spiral Reverse Osmosis Membrane System

Experimental particle physics introduction

Section 1: Cell Membrane