1 / 21

TEMPO ® Pre-work Chapter 1 – System Introduction

TEMPO ® Pre-work Chapter 1 – System Introduction. Objectives . Upon completion of this chapter , you will be able to: Explain the TEMPO ® Theory of Operation Identify the TEMPO consumables. Food Quality.

pello
Download Presentation

TEMPO ® Pre-work Chapter 1 – System Introduction

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. TEMPO® Pre-work Chapter 1 – System Introduction

  2. Objectives • Upon completion of this chapter, you will be able to: • Explain the TEMPO® Theory of Operation • Identify the TEMPO consumables

  3. Food Quality Under specific government guidelines, the agri-food industry routinely monitors the quality of the entire production process, from raw materials to finished products.

  4. Food Quality To determine the quality of a food sample, we count the number of specific microorganisms within a sample. A microorganism is a small (micro) living system (organism) such as bacteria or parasites.

  5. Food Quality Scientist have determined what microorganisms we should test for (quality indicators). These Quality Indicators are of major importance in guaranteeing the commercial value of finished products, from their exit of the plant to the end of their shelf life.

  6. What is the TEMPO®? The TEMPO is an automated testing system used to count (enumerate) the number of specific microorganisms (quality indicator) in food and environmental samples.

  7. Methods Several methods can be used to enumerate the quality indicators within a sample. The TEMPO® is based on the Most Probable Number (MPN) method.

  8. 3 x 1 mL 3 x 1 mL 10-3 10-4 The Most Probable Number The principle of the MPN method is to allow any microorganism present in samples to grow in suitable conditions in tubes, using a minimum of three dilutions and three tubes per dilution. Food 10g Diluent 90mL 3 x 1 mL 9 mL diluent 10-2 Homogenizer Bag 10-1 suspension Traditional manual method

  9. Colony Forming Unit / Gram Colony Forming Units per gram (CFU/g) - When we count microorganisms in a sample, we dilute the food to a suitable level then mix it with culture medium. Microbiological counts work on the principle that each single bacteria present will grow to produce a colony which will be visible to the naked eye after 24 to 48 hours (longer for yeasts and molds). Then the number of colonies are counted, multiplied by the dilution factor and this gives us a count per gram of the organisms present.

  10. 3 x 1 mL 3 x 1 mL 10-3 10-4 Result = 2,1.104 CFU/g 3 / 2 / 2 2 + 3 + 2 + The Most Probable Number 3 x 1 mL Homogenizer Bag 10-1 suspension 9 mL diluent 10-2 Incubation Most Probable Number MPN Statistic Table Traditional manual method

  11. The TEMPO® Consumables • The card is the heart of the TEMPO system, offering the precision of the MPN method in 16 tubes without the usual tedious preparation. • Each card includes a transfer tube, and a barcode with a unique identification number, the type of test and a variety of other information.

  12. The TEMPO® Consumables Each single-use vial of TEMPO media contains a fluorescent indicator (4MU), powder culture medium required for the enumeration of quality indicators and a barcode with a variety of information.

  13. How the TEMPO® does it The TEMPO system automates the manual MPN method by using a preparation station to prepare the TEMPO cards…

  14. How the TEMPO® does it … and a reading station to read and analyze the cards.

  15. The TEMPO® Consumables • The TEMPO bag: (Homogenizer Bag) • To blend the food sample with the diluent • To filter the sample • Single-use consumable

  16. Step 1: Sample Preparation TEMPO® workflow Diluent 90mL Food 10g Homogenizer Bag • The processed food sample is poured into the vial containing the dehydrated media • The vial and card are placed in the 6-card filling rack

  17. Single use vial with a specific dehydrated medium Card TEMPO® workflow Step 2: CardFilling • The card is filled with the mixture of medium and sample • The straw is automatically cut and sealed by the instrument

  18. Comparison of dilutions used in the manual MPN method How the TEMPO® does it The TEMPO utilizes a plastic card and performs three different dilutions in 48 wells (3 x 16). Small wells (2.25µl) Medium wells (22.5 µl) Large wells (225µl)

  19. TEMPO® workflow Step 3: Incubation The cards are transferred to a 20-card rack for incubation

  20. TEMPO® workflow Step 4: Automatic reading and interpretation The operator places the incubated 20-card rack into the reader

  21. Summary and Questions In this chapter, you were introduced to the TEMPO®. You learned what the TEMPO is and how it works. Before we move on to the next chapter, does anyone have any questions? If so, call Luke Bailey at 919-620-2285 with any questions.

More Related