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EasySpray Concept Products

EasySpray Concept Products. Ole Vorm April 2010. EasySpray Products Introduction. Background:

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EasySpray Concept Products

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  1. EasySpray Concept Products Ole Vorm April 2010

  2. EasySpray Products Introduction Background: • Nano-flow LC-MS is used increasingly by non-experts. It is a frequent observation that users obtain very poor LC-MS results owing to an unstable spray with leaks and dead volumes. This is because they cannot properly connect column and emitter and spray voltage and subsequently position the assembly in front of the MS inlet orifice in working order. • For this reason, the plug-and-play solution provided by the Agilent Chip Cube product has gained popularity despite great disadvantages such as low loading capacity, high retention time jitters, poor resolving power, and high costs. • EasySpray provides an equally simple plug-and-play solution while having none of the mentioned disadvantages. • The concept consists of a source and a range of columns with permanently attached emitters and pre-made high voltage connection. The source and column/emitter have a “snap fit” functionality. • Patent application has been submitted to cover method and product. Products: • A series of sources can be made to fit all mass spectrometer brands. Bruker has already expressed a keen interest in getting access to the products for their MS systems. We should of course consider whether to keep this proprietary to Thermo. • A range of column sizes and types will be made. Each column can be used on any EasySpray source. Any column up to OD 1/16” can easily be turned into an EasySpray column by the addition of emitter/fitting and subsequent embedding in resin.

  3. EasySpray Product Prototype Photos Photo of prototype EasySpray source installed on a Thermo TSQ mass spectrometer. The spray chamber is fully enclosed and provides no access to touch high voltage points. X-Y-Z manipulation is not required once a (mass spectrometer specific) optimal spray position has been identified. Emitter tip position is the same for every column/emitter.

  4. EasySpray Product Photos Cross section of emitter/column assembly. Columns up to OD 1/16” will be sleeved to fit. Emitter is35mm long and made of steel OD 150µm, ID 25 µm or etched fused silica of similar dimensions. Schematic of outer shape. Black colour is polyurethane resin for isolation. Orange colour is 316 stainless steel for spray voltage.

  5. Packed Emitter vs Direct Junction Pulled & packed emitter 15 cm column, 30 min gradient FWHM 5,4 sec EasySpray Column FWHM 5,1 sec

  6. EasySpray elution profiles 300nl/min, 50fm BSA, 75µm ID, 1.9 µm particle size, 1column setup 10nl (2.1 sec) 100 nl/min, 50 fm BSA , 75 µm ID, 1.9 µm particle size 1 column setup 6 nl (3.6sec)

  7. Direct Infusion at Univ. Southern Denmark Using Prototype EasySpray Setup m/z 433 (3+) Extracted ion Currents of Angiotensin 1 (2+ and 3+ ions) in optimized position then sprayer was unplugged and plugged in again. No change in ion intensity observed! m/z 649 (2+)

  8. Direct Infusion at Novo Nordisk Pharmaceuticals Using Prototype EasySpray Setup m/z 433 (3+) Extracted ion Currents of Angiotensin 1 (2+ and 3+ ions) with Infuser 3 in optimized position and infuser 4 in the same position. Two different sprayers in the same position have virtually same performance. Note: outliers still occur. Exact intensity RSD under investigation. m/z 649 (2+)

  9. Repeat Injections of BSA at Novo Nordisk Pharmaceuticals Using Prototype EasySpray Setup (and Easy-nLC)

  10. EasySpray Key Financials • Razor Blade business model (presumably). • Source COGS ≈ $1,000 • Expected sales price $3k - $15k • Column COGS ≈ $50 • Expected sales price $500 - $1200 • Column consumption per LC setup should be around 1 column per two weeks • 24 columns per year = $12,000 per year and LC • Extremely high GM and significant competitive advantage

  11. EasySpray Launch • Launch postponed from ASMS 2010 to HUPO at the earliest • Launch at HUPO requires decision soon and support from multiple groups outside Odense. • Initial column selection will be small: e.g. C18, 75µm ID, 3 µm beads, Lengths of 10 cm, 20 cm, and 30 cm. • Expansion of column range begins in Q3 or Q4 of 2010. • External user tests are underway at Novo Nordisk (big-pharma R&D group) and SDU (academic R&D group, LC-MS experts) • Initial results from internal and external tests confirm high performance and ease-of-use. • 0-series of 20 sources will be received around June 7 • Processes for scaling up column production will be finished end of May

  12. EasySpray, additional prototype photos

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