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The Cell Cycle

Explore the reasons why prokaryotic cells do not undergo the same cell cycle as eukaryotic cells. Understand the stages of the cell cycle, including DNA replication and mitosis, and learn how to experimentally prove DNA replication occurs during the S phase. Examine the role of chromosomes and kinetochores in the cell cycle, and discover the implications of differences in cell division for evolution. Gain insights into the regulation of the cell cycle and its significance in cancer development.

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The Cell Cycle

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  1. Chapter 12 The Cell Cycle Questions prepared by Louise PaquinMcDaniel College

  2. Prokaryotic DivisionWhy don’t prokaryotic cells need to go through the same cell cycle as eukaryotic cells?

  3. S PhaseHow might you prove experimentally that DNA replication (and therefore the replication of chromosomes) happens during the S phase of the cell cycle and not during mitosis?

  4. Chromosomes in the Cell CycleAt what part of the cell cycle would you see a chromosome that looks like this? Give your reasons.

  5. EvolutionWhat do the differences among the division of these cells demonstrate?

  6. Kinetochore Which of the following best describes the kinetochore? • a structure composed of several proteins that associate with the centromere region of a chromosome and that can bind to spindle microtubules • the centromere region of a metaphase chromosome at which the DNA can bind with spindle proteins • the array of vesicles that will form between two dividing nuclei and give rise to the metaphase plate • the ring of actin microfilaments that will cause the appearance of the cleavage furrow • the core of proteins that forms the cell plate in a dividing plant cell

  7. Rate of MitosisIf you were to examine a sample of connective tissue cells from a small but adult mammal and a second sample from a fetus of the same species, in which would you expect to find more cells undergoing mitosis? Why?

  8. Regulation of the Cell CycleA. What would you expect to happen if you fuse a cell in S with a cell in G1? Why?B. What would you expect to happen to a nucleus from a G1 cell if it is introduced into an enucleated M cell? Why?

  9. RegulationWhich of the following would need to be present to produce a realistic signal to control the cell cycle? (More than one may be required.) • a set of molecules that are sensitive to fluctuations in the abundance of regulators • protein kinases • molecules that activate kinases • molecules that degrade activators • All of the above.

  10. Cancer CellsAt which part of the cell cycle would you expect that a change in regulation could result in cancer? Provide a reason for your answer.

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