1 / 1

Adaptor sequence primer : 5’- GCG AGC ACA GAA TTA ATA CGA CT -3’ ORF C68 and ctRNA1

Table 1. Oligonucleotides used in RT-adaptor PCR and primer extension experiments. Adaptor sequence primer : 5’- GCG AGC ACA GAA TTA ATA CGA CT -3’ ORF C68 and ctRNA1 ORF C68 5´-ATG AGA CCA GGC ATA CGC AAA CTA GTG-3´

petra
Download Presentation

Adaptor sequence primer : 5’- GCG AGC ACA GAA TTA ATA CGA CT -3’ ORF C68 and ctRNA1

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Table 1. Oligonucleotides used in RT-adaptor PCR and primer extension experiments Adaptor sequence primer: 5’-GCG AGC ACA GAA TTA ATA CGA CT -3’ ORFC68 and ctRNA1 ORFC68 5´-ATG AGA CCA GGC ATA CGC AAA CTA GTG-3´ ORFC68ter15- GCGAGCACAGAATTAATACGACTTATCAAAAAAACACGTAACTTAAATTT -3´ ORFC68ter25´-GCGAGCACAGAATTAATACGACTTTATATTATATACGGGTCTAGTTTTT-3´ ORFC68ter35´-GCGAGCACAGAATTAATACGACTCCTTCTTTCCCTTTCTGTTTTCTTA -3´ ORFC68ter45´-GCGAGCACAGAATTAATACGACTGTGACTTTTCTTACACTTCTCT-3´ ORFc68ter55´-GCGAGCACAGAATTAATACGACTACGTAAGCGAAAGCCTGAAATAACGGAAAAAT -3´ ORF76 Up76-5’-GTCAAACGTTAGAGGAGTTAGAAGAG-3’ ORF76-end-5´-GCGAGCACAGAATTAATACGACTCTAGTCGGCTAATTCATCTCTATG-3´ ORF76/154-5´-GCGAGCACAGAATTAATACGACTCTCACGTCTCAACTCTTCTATATCA-3´ ctRNA3 ctRNA3up 5’- AAGATATAGTAAGAAGAATTGGTAAT-3’ ctRNA3dw1- 5’-GCGAGCACAGAATTAATACGACTCAGCTAATC CTAATCCGTTTGAGTAAT -3’ ctRNA3dw2- 5’-GCGAGCACAGAATTAATACGACTGCGAAAGATATTTAACATTCTGTTA ORF892 MDORF892- 5´-GACTTGCATAATGAATTAACA AAG AAC ATA GCT AC-3´ ORF892end-5´-GCGAGCACAGAATTAATACGACTTTACCCGGCTAATATTCTGAATTGA-3´ ORF892ter1-5´-GCGAGCACAGAATTAATACGACTTAATTACAGATGAAAAATTGAATTTCAAAAAAT –3´ ORF892ter2-5´-GCGAGCACAGAATTAATACGACTATTAAAAGAAAAAAAATCCGGATTTCGGTG-3´ ORF892ter3-5´-GCGAGCACAGAATTAATACGACTGCTATTTTTACCCCTATACACACATGC -3´ ORF288 and Tn ORF288LF- 5’-GAATTAGTGG GGAAACCAAC AAC-3’ ORF288end-5´-GCGAGCACAGAATTAATACGACTTTATTTCAGGCTTTCGCTTACGTTA-3´ ORF288ter15´-GCGAGCACAGAATTAATACGACTAGTCACGTGCCTCTTTATTCTTATTT-3´ ORF288ter25´-GCGAGCACAGAATTAATACGACTAGAAGGGCGTAAAATAACGGGTAAGA-3´ ORF288ter35´-GCGAGCACAGAATTAATACGACTTAAAAAACTAGACCCGTATATAATATAATAAGA-3´ ORF288ter4 5´-GCGAGCACAGAATTAATACGACTATGGGGATATTGTGTTATCGTATAAGAGGG -3´ ORF288ter55-GCGAGCACAGAATTAATACGACTATGAGACCAGGCATACGCAAACTAGTG-3´ ORF288ter6-5´-GCGAGCACAGAATTAATACGACTGGGTATTACCCCGTGGCTTTTCGTATG-3´ ORF288ter7-5´-GCGAGCACAGAATTAATACGACTTTTTGTCACCAGGTACAGTATAGT -3´ ctRNA2 ctRNA2 5´-ATGTGCGGAAGAGACTAGAGTGACATC-3’ ctRmRNA1- 5´-GCGAGCACAGAATTAATACGACTCTTTCTGTACATATGACAAACATATCA-3´ ctRmRNA2- 5´-GCGAGCACAGAATTAATACGACTGGAGGCGAACCAGGTGTCTAAT-3´ ORF91 MidwnORF91 5’-CAAACATATCAAACACGCTTCTCATAAAAATGC-3’ ORF91a 5´-GCGAGCACAGAATTAATACGACTCTTCTCTGATCTCACTGTAGATCCT-3´ ORF91b 5´-GCGAGCACAGAATTAATACGACTCATGATCTCGCCACCCTCAAGACCTTGA-3´ ctRNA3prext 5’- GACTTCACCAGAGAGGCGTGATTTATCGT-3’ RNA4prext5’- AAAAAAACTAGACCCGTATATAATATAATAAG-3’

More Related