Refining

1. Ethanol Still Bottom Water Cumulative Cash Flow Basis: 1% of Market Glucanex Glucose Water Water Antifoam Vent Cumulative Cashflow Sterile air Fermentation Dissolved Air Floatation CO2 Lysing Centrifuge Dryer Algae Algae Cake to Wholesaler Salt Algae Growth Cycle Year of Plant Life 30 CIP System Steam Cooling Water Air CO2 25 20 Cell Density (g/L) 15 10 Sodium methoxide Citric Acid Soft Water Refrigerant 5 0 0 20 40 60 80 100 Interesterification Emulsification Pasteurization Scraped Surface Heat Exchanger Time (Hours) Gum Water Citric Acid Acid Activated Clay 7 • Infant Health • Adult Health Sterile Glucanex Sterile Anti-foam Filter 3 Degumming Centrifuge Bleaching Removal FFA Deodorization Water 11 • Lack of DHA is associated with poor or slower retinal • development and brain development • Helps infants gain weight faster • Recommended 300 mg/day for pregnant or lactating • women • A lack of DHA is associated with Alzheimer’s disease • Inhibits the growth of human colon carcinoma cells • Increases the efficacy of chemotherapy P- 6 HX-1 Crude SBO Packaging Steam 10 Sea Salt QIC QIC QIC P- 3 Hardfat SBO Lecithin PIC Vented Air Filter 2 Sodium methoxide Foam Diacetyl 13 Visc Steam Mono/Diglycerides Main Fermenter Citric Acid Citric Acid Citric Acid To Wholesaler Turbidity Soft Water B-Carotenes QIC Enzyme Acid Activate Clay QIC Sorbic Acid 14 9 P- 8 NaOH Ergocaliferols QIC TIC Cooling Water Sparged Steam 8 O Filter 1 Compressor 1 TIC Sterile Air Air FIC PIC HO Cumulative Cashflow 0g Trans Fat 100% Vegan Premium Golden Spread Cumulative Cashflow Algae Oil to a Functional Food Just Spread It On! Travis Dallas Eric Graves Joaquin Martinez Chris McNinch Ramune O. Meskyte Charu Saini Mentors: Dan Rusinak, Jeffery Perl, John Micheli Crypthecodinium cohnii:A natural DHA Factory • 20 % lipid content by weight which is 40 % DHA • Facultative heterotroph, which does not perform • photosynthesis • Growth rate of 0.0375 h-1 on glucose substrate • Uses glucose as a carbon source • Uses dead yeast from ethanol bottoms as a nitrogen • source • Requires dissolved oxygen for optimal growth • Grows in brackish (saline) water. Growth Phase Maintenance Docosahexaenoic Acid Algae Oil Fermentation Controls 35,000 gallon Fermentor Height 50 feet Diameter 12 feet C22 H32 O2 You should get DHA from algae just like me. It is tons healthier for both of us. Alpha Algae Co. Truck Engines Alpha Algae Co. Alpha Algae Co. 1928 SE Otis Rd. Cedar Rapids, IA 52401 Margarine Fermentation Refining Eat Premium Golden rather than me for your essential fatty acids. Butter your biscuits! Cumulative Cash Flow Basis: 2% in 6 Years Expand to 2% Market Share in 6 Years Payback Period 7.13 Years Net Present Value 158,394,244.01 USD Internal Rate or Return 34.5% What is Sanitary Design? Preventing microbial growth and simply being clean How do you go about this? Physical separations of the different plants All equipment composed of stainless steel Margarine Plant composed of polished stainless steel Linear and horseshoe shaped plant design Clean in Place system (CIP) on the inside Spray cleaning foam on the outside Who cares? You do, and so do we. We do not want you getting sick from our product. We like it clean! Year of Plant Life • Total Project Capital Cost • 37,328,232.77 USD • Operating Costs18,962,032.85 USD/Year • Raw Materials 6,031,280.00 USD/Year • Utilities 3,532,157.83 USD/Year • Maintenance 2,030,000.00 USD/Year • Operating Labor Costs 2,828,000.00 USD/Year • Operating Charges 707,000.00 USD/Year • Plant Overhead 2,429,000.00 USD/Year • G and A Costs 1,404,594.96 USD/Year Total Product Sales 25,963,238.43 USD/Year Payback Period 5.7 Years Net Present Value 84,727,200.17 USD Internal Rate or Return 25.5% Cumulative Cash Flow Basis: 2% of Market 2% Market Share from Start Payback Period 5.00 Years Net Present Value 198,627,853.09 USD Internal Rate or Return 34.5% Sooo Healthy! 0g Trans Fatty Acids 100% Vegan 100% Daily Omega-3 Fatty Acids per Serving INGREDIENTS: INTERESTERIFIED SOYBEAN OIL, WATER, ALGAE OIL, NATURAL SEA SALT, MONO/DIGLYCERIDES, NATURAL LECITHIN, SORBIC ACID, BETA-CAROTENE (VITAMIN A), ERGOCALCIFEROL (VITAMIN D), DIACETYL (FLAVORING) Year of Plant Life