1 / 6

Aggrecan and its role in bone development

Aggrecan and its role in bone development. Aryn Gabai. Role of Aggrecan. Very large (200+ kDal) structural protein Links ECM components, compression resistant – essentially a ECM scaffold Connects hyaluronic acid and link protein for bone mineralization

phuoc
Download Presentation

Aggrecan and its role in bone development

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Aggrecan and its role in bone development Aryn Gabai

  2. Role of Aggrecan • Very large (200+ kDal) structural protein • Links ECM components, compression resistant – essentially a ECM scaffold • Connects hyaluronic acid and link protein for bone mineralization • We have observed that PERK mice have a reduced amount of aggrecan in the total growth plate • Difficulty with identifying causes for reduced amount – smaller gp? less aggrecan? reduced chondrocytes?

  3. 7293 KO 19d 7289 WT 19d 12915 WT 17d 7296 KO 19d 7289 WT 19d 12913 KO 17d 7297 WT 19d 12916 HT 17d 7296 KO 19d

  4. Isolated aggrecan for REX chondrocytes • Shows a working aggrecan western ------- 5 uL-------- ------ 10 uL-------

  5. Tissue-based westerns are not as clear or quantifiable 15d HT 15 d WT 15d KO 15d HT 15d HT 15d HT 15d KO 15d WT 17d KO 17d HT REX 17d HT

  6. Project Future • Is this data enough to continue researching this protein? • Identify housekeeping protein to quantify western results • More western replication

More Related