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DNA

DNA. Deoxyribonucleic Acid. Defined : Molecule that stores genetic information Nucleotides: Three repeating units of nucleic acids 1) Sugar 2) Phosphate Group 3) Nitrogen Base Millions of nucleotides per strand of DNA. How many Nucleotides?. Nucleotides.

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DNA

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  1. DNA

  2. Deoxyribonucleic Acid • Defined: Molecule that stores genetic information • Nucleotides: Three repeating units of nucleic acids • 1) Sugar • 2) Phosphate Group • 3) Nitrogen Base • Millions of nucleotides per strand of DNA

  3. How many Nucleotides?

  4. Nucleotides • Sugar (Deoxyribose) connects with the phosphate group • 4 Different Bases: • Adenine (A) • Thymine (T) • Guanine (G) • Cytosine (C) • Rule: A always joins with T • Rule: C always joins with G • Human DNA: ~3 billion pairs C T A A T G T

  5. Double Helix • James Watson and Francis Crick identified DNA’s structure in 1953 • Double Helix: spiral shape of DNA • Left and right nucleotide strands are connected by a hydrogen bond

  6. DNA Replication • Defined: Process of copying DNA • During interphase (S-stage) • Step 1: DNA Helicase “unzips” the two DNA strands (breaks the H bonds) • Step 2: Free floating nucleotides (A, T, C, G) match up • Step 3: DNA Polymerase reconnects the two strands

  7. DNA Polymerase Glues the DNA T A A T G C G C Two Identical Strands of DNA! A T C G T A A T G C G C C G T A C G A T DNA Helicase Splits the DNA

  8. Accuracy & Repair • Mutation: change in DNA sequence • Mutations can be corrected • Enzyme “proofreads” DNA and check/correct errors • Pre-repair: 1 in 10,000 nucleotides has an error • Post-repair: 1 in 1 billion nucleotides has an error

  9. Quick Review • DNA nucleotides form long chains • DNA nucleotides have 4 different bases (A, T, C, G) • DNA makes exact copies of itself

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