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hypotonic. Osmoregulation. Water balance vs. Habitat freshwater hypotonic to body fluids water flow into cells & salt loss saltwater hypertonic to body fluids water loss from cells land dry environment need to conserve water may also need to conserve salt. hypertonic.
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hypotonic Osmoregulation • Water balance vs. Habitat • freshwater • hypotonic to body fluids • water flow into cells & salt loss • saltwater • hypertonic to body fluids • water loss from cells • land • dry environment • need to conserve water • may also need to conserve salt hypertonic Why do all land animals have to conserve water? • always lose water (breathing & waste) • may lose life while searching for water
H O H | | | –C– C–OH N | H R Intracellular Waste • What waste products are made inside of cells? • what do we digest our food into… • proteins = CHON • nucleic acids = CHOPN lots! verylittle CO2 +H2O + N CO2 +H2O + P + N CO2 + H2O NH2= ammonia
Nitrogenous waste disposal • Ammonia (NH3) • very toxic • carcinogenic • very soluble • easily crosses membranes • must dilute it & get rid of it… fast! • How you get rid of nitrogenous wastes depends on • who you are (evolutionary relationship) • where you live (habitat) aquatic terrestrial terrestrial egg layer
Nitrogen waste • Aquatic organisms • can afford to lose water • ammonia • most toxic • Terrestrial • need to conserve water • urea • less toxic • Terrestrial egglayers • need to conserve water • need to protectembryo in egg • uric acid • least toxic
Freshwater animals • Hypotonic environment • water diffuses into cells • Manage water & waste together • remove surplus water & waste • use surplus water to dilute ammonia & excrete it • also diffuse ammonia continuously through gills • overcome loss of salts • reabsorb in kidneys or active transport across gills
H H H H N N C O Land animals • Nitrogen waste disposal on land • need to conserve water • urea= larger molecule = less soluble = less toxic- produced in liver • kidney • filter solutes out of blood • reabsorb H2O (+ any useful solutes) • excrete waste • urine = urea, salts, excess sugar & H2O • urine is very concentrated • concentrated NH3 would be too toxic mammals
Egg-laying land animals • Nitrogen waste disposal in egg • no place to get rid of waste in egg • need even less soluble molecule • uric acid = BIGGER = less soluble = less toxic • birds, reptiles, insects
O O O N N N N H H H H Uric acid • Polymerized urea • large molecule • precipitates out of solution • doesn’t harm embryo in egg • white dust in egg • adults still excrete N waste as white paste • no liquid waste • uric acid = white bird “poop”!
Mammalian System blood filtrate • Filter solutes out of blood & reabsorb H2O + desirable solutes • Key functions • filtration • fluids (water & solutes) filtered outof blood • reabsorption • selectively reabsorb (diffusion) needed water + solutes back to blood • secretion • pump out any other unwanted solutes to urine • excretion • expel concentrated urine (N waste + solutes + toxins) from body concentratedurine
Nephron: Filtration • At glomerulus • filtered out of blood • H2O • glucose • salts / ions (Na+ / Cl–) • urea • not filtered out • cells • proteins high blood pressure in kidneysforce to push (filter) H2O & solutes out of blood vessel
Nephron: Re-absorption • Proximal tubule • reabsorbed back into blood • NaCl • active transport of Na+ • Cl– follows by diffusion • H2O • glucose • HCO3- • bicarbonate • buffer for blood pH
Nephron: Re-absorption • Loop of Henle • descending limb • reabsorbed • H2O • structure • many aquaporins in cell membranes • high permeability to H2O • no Na+ or Cl– channels • impermeableto salt
Nephron: Re-absorption • Loop of Henle • ascending limb • reabsorbed • Na+ & Cl– • structure • many Na+ / Cl– channels in cell membranes • high permeability to Na+ & Cl– • no aquaporins • impermeable to H2O
Nephron: Re-absorption • Distal tubule • reabsorbed • salts • H2O • bicarbonate • HCO3- • regulate blood pH
Nephron: Reabsorption & Excretion • Collecting duct • reabsorbed • H2O = through aquaporins • excretion • concentrated urine • to bladder • impermeable lining = no channels in cell membranes