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Microscopes

Microscopes. “micro” means small ! “scope” means watch/look !. Fun Fact! Microscopes were first developed as entertainment for the rich!. Microscopy. What’s magnified?. Apple Orange Fingertip Cardboard Gum Shirt. Apple!. What’s magnified?. Apple Orange Fingertip Cardboard Gum

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Microscopes

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  1. Microscopes • “micro” means small! • “scope” means watch/look! Fun Fact! Microscopes were first developed as entertainment for the rich!

  2. Microscopy

  3. What’s magnified? • Apple • Orange • Fingertip • Cardboard • Gum • Shirt Apple!

  4. What’s magnified? • Apple • Orange • Fingertip • Cardboard • Gum • Shirt Shirt!

  5. What’s magnified? • Apple • Orange • Fingertip • Cardboard • Gum • Shirt Gum!

  6. What’s magnified? • Apple • Orange • Fingertip • Cardboard • Gum • Shirt Fingertip!

  7. Light Microscopes • Focuses visible light rays • Can be used on live or dead specimens • Dyes & fluorescent markers help for viewing • Magnify up to 1000X on the ones we have! • Compound Light Microscopes

  8. Light Micrographs Plant Human Chromosomes 

  9. The Compound Light Microscope The word compound means to increase! A compound microscope has 2 lenses to increase magnification!

  10. Parts of the Microscope • Ocular Lens: eyepiece (10x magnification), first lens • Arm: connects base & body tube • Must hold arm while carrying microscope! • Course Adjustment Knob: roughly focuses object • Used to find specimen

  11. Parts of the Microscope 4. Fine Adjustment Knob: small knob, finely focuses specimen • Use after object has been found! 5. Power Cord: must be wrapped around arm after use 6. Nosepiece: holds 3 objective lenses

  12. Parts of the Microscope 7. Objective Lens: second lens • Scanning Lens (4x) • Low Power (10x) • High Power (40x) • Oil Immersion (100x)

  13. Parts of the Microscope 8. Stage Clips: holds slide in place 9. Stage: flat plate where slides are held 10. Diaphragm: regulates the amount of light exposed to the specimen

  14. Parts of the Microscope 11. Condenser Lens: focuses light on specimen 12. Power Switch: turns light on & off 13. Base: support of the microscope

  15. Field of View • Total area visible through the ocular lens The higher the magnification, the smaller the field of view!

  16. Total Magnification Total Magnification = ocular lens x objective lens If the 40x objective is in place… TM = 400x, because (10 x 40 = 400)

  17. Focusing the Microscope • Locate the slide specimen at scanning power. • Then move the area to be viewed to the center of the field of view. • Make sure the slide is in perfect focus at scanning power before moving up to low power. • Turn your objective without moving the slide or stage. • You will slightly adjust the image at the next objective. • At high power use only the fine focus knob and do not turn it more than 1/2 turn.

  18. Important! Microscope Rules • Always carry with 1 hand on arm & 1 hand on base! • Use a slide & coverslip. • Clean Up: • Always return to scanning (lowest) power! • Always lower stage all the way! • Unplug, wrap cord, & cover

  19. Intro. Microscope Lab NO HORSEPLAY!! • Follow the directions carefully… don’t skip over things just because you don’t feel like reading!

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