1 / 17

Fish Heath Management Lab 5: Histology (microanatomy)

Fish Heath Management Lab 5: Histology (microanatomy). Tarah Johnson March 2 nd , 2009. Objectives. Be able to microscopically identify cells and structures in the liver, kidney, and gills

raisie
Download Presentation

Fish Heath Management Lab 5: Histology (microanatomy)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Fish Heath Management Lab 5: Histology (microanatomy) Tarah Johnson March 2nd, 2009

  2. Objectives • Be able to microscopically identify cells and structures in the liver, kidney, and gills • Have the opportunity to microscopically examine histological sections of other fish organs (eyes, head, muscle, etc.)

  3. Liver • Hepatocytes • Hepatic Sinusoid • Central Vein and or Hepatic Portal Vein • Bile Duct • Arteriole • Glycogen Vacuole

  4. Central Vein and Hepatic Sinusoids Hepatic sinusoids Erythrocytes visible within the central vein 400x

  5. 400x 400x A Bile Duct Arteriole A. Glycogen Vacuole Erythrocytes visible within arteriole

  6. Head Kidney • Interrenal gland • Chromaffin cell • Postcardinal vein • Hematopoietic tissue

  7. 400x A B C • Interrenal Gland • Chromaffin Cell • Postcardinal vein

  8. 40x A B A. Postcardinal vein B. Hematopoietic tissue

  9. Trunk Kidney • Nephron (may not observe a complete nephron): Renal corpuscle, proximal tube, distal tubule, and collecting duct • Renal Corpuscle:Glomerulus, Bowman’s capsule, basement membrane of glomerulus • Melanocytes • Collecting duct and/or Mesonephric duct • Connective tissue

  10. 400x • Renal Corpuscle • Proximal Tubule • Distal Tubule C B A

  11. 400x • Glomerulus • Basement membrane of glomerulus • Bowman’s capsule C B A

  12. 400x C A B • Mesonephric Duct • Melanocytes • Connective tissue

  13. Gills • Filament • Lamella • Goblet (or mucous) cell • Chloride cell • Pillar (or pilaster) cell • Gill filament cartilage

  14. Lamella B C • Filament cartilage • Pillar cell • Chloride cell A 400x Gill filaments and lamellae 40x

  15. 400x Mucous or Goblet Cells

  16. Laboratory Steps • Use proper microscope handling procedures (distributed in Lab 2) • Examine histology slides of liver, kidney, and gills • Examine additional histology slides of other organs

  17. Be Sure to… • Clean oil immersion lenses • Clean slides and return to proper case (the same one you took it from) • Turn off microscopes and leave on low power objective when done

More Related