1 / 16

p57: Beckwith-Wiedemann Syndrome

p57: Beckwith-Wiedemann Syndrome. Presented By: Jameeka Carrington. Symptoms of BWS. Large body size (macrosomia) Large tongue (macroglossia) Large organs (visceromegaly) Abdominal wall defects (i.e. umbilical hernia ) Hypoglycemia (low blood sugar). Symptoms of BWS.

ralph
Download Presentation

p57: Beckwith-Wiedemann Syndrome

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. p57: Beckwith-Wiedemann Syndrome Presented By: Jameeka Carrington

  2. Symptoms of BWS • Large body size (macrosomia) • Large tongue (macroglossia) • Large organs (visceromegaly) • Abdominal wall defects (i.e. umbilical hernia ) • Hypoglycemia (low blood sugar)

  3. Symptoms of BWS • Large prominent eyes • Creases in ear lobes • Undescended testicles • Seizures

  4. Symptoms of BWS • Metopic Ridge- a ridge of bone or suture line on the forehead between the two halves of the frontal bone. The ridging is caused when the two halves close prematurely. www.nlm.nih.gov/medlineplus/ency/article/001186.htm

  5. Symptoms of BWS • Microcephaly- abnormal smallness of the head • Macroglossia- enlarged tongue • Umbilical hernia- protrusion of the intestines through the abdominal wall in the navel region www.nlm.nih.gov/medlineplus/ency/article/001186.htm

  6. Symptoms of BWS • Increased predisposition to tumor development • Wilms’ tumor • Adrenocortical carcinoma • Rhabdomyosarcoma • Hepatoblastoma

  7. Tests for BWS • Bone X-ray • Blood tests for low sugar • Ultrasound of the abdomen • X-ray of the abdomen • MRI of the abdomen • Chromosome studies

  8. Genetic Basis of BWS • 85% of cases are sporadic • Inherited in autosomal dominant fashion • Mapped to chromosome 11p15 • Translocation breakpoints found within chromosome map to three distinct regions • Region 1, BWSCR1, contains 5 translocation breakpoints, which all disrupt the KCNQ1 gene and is the region of primary concern

  9. Chromosome 11 Steenman et al. (2000) Genes, Chromosomes & Cancer 28:2.

  10. p57 • Member of the Cip/Kip family of mammalian CKI’s along with p21 and p27 • Differs from p21 and p27 in structure by insert of proline/alanine rich or acidic motifs following the Cdk inhibitory domain • Inhibits G1 cyclin-Cdk complexes by binding to cyclin and blocking the catalytic site of the associated Cdk • Expressed during embryonic development

  11. IGFII • Insulin-like growth factor II, or IGFII, is a single chain polypeptide that is 47% homologous with insulin • Functions • Mediates growth hormone action • Stimulates the growth of cultured cells • Stimulates the action of insulin • Involved in development and growth • Autocrine regulator of cell proliferation

  12. Imprinting of p57 and IGFII • p57 is paternally imprinted in the genome • IGFII is maternally imprinted in the genome • Genomic imprinting is the reversible modification of DNA that causes differential expression of maternally or paternally inherited genes • A gene which is imprinted, is inactivated, by being methylated • Imprinting suppresses gene transcription and takes place during gametogenesis • Chromosome 11 is one of only nine chromosomes that are suspected to have imprinted regions

  13. p57 and IGFII Act as Antagonists • Several studies have also shown that IGFII expression down regulates the activity of p57 • p57 is a negative regulator of cell proliferation • IGFII is a positive regulator of cell proliferation • Both over expression of IGFII and inhibited expression of p57 result in BWS symptoms • Together, p57 and IGFII act antagonistically Grandjean et al. (2000) PNAS 97:5281.

  14. Loss of Imprinting (LOI) and BWS • Imprinting defects of IGFII is the most prominent cause of the development of BWS • LOI of IGFII results in biallelic expression of IGFII, which down regulates expression of p57 at an increased level • Paternal duplication of IGFII and the presence of a defective maternal p57 allele contributes to the development of BWS as well Weksburg et al. (2001) Human Molecular Genetics 10:2989-3000.

  15. Two separate studies were conducted using mutant mice carrying deletions of the beginning of the p57 gene Resultant defects common between both studies correlate with symptoms of BWS p57 and BWS Swanger, W. Jherek, Roberts, James M. (1997) BioEssays 19:840.

  16. Summary • BWS is an autosomal dominant disorder characterized by overgrowth and predisposition to tumor development • p57 and IGFII, both located on chromosome 11, are believed to be highly associated with the development of BWS • Defects in the imprinting of p57 and IGFII have been experimentally shown to reproduce BWS symptoms in mutant mice

More Related