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Garlic & Health: Understanding Allicin Formation Pathway

This project aims to unveil the biochemical and physiological steps leading to allicin production in garlic. By analyzing the pathway integration with development and genes involved, it sheds light on the biochemistry of garlic sulfur compounds. The project employs HPLC and HPLC-MS to identify intermediates and isolates alliinase clones for in-depth sequencing. Milestones include identifying pathway intermediates and establishing the first sulfur budget for garlic.

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Garlic & Health: Understanding Allicin Formation Pathway

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  1. GARLIC & HEALTH • P2 HRI Wellesbourne • Brian Thomas • Lol Trueman • Brian Smith • P3 University of Liverpool • Hamish Collin • Meriel Jones • Brian Tomsett WP4 Garlic Sulphur Biochemistry

  2. GARLIC & HEALTH • To establish the limiting biochemical and physiological steps for allicin formation in garlic • Biochemical pathway • Integration with development • Genes for limiting steps Garlic Sulphur Biochemistry

  3. GARLIC & HEALTH Pathway Biochemistry

  4. GARLIC & HEALTH Pathway Biochemistry (II) CSO3 Alliinase Tissue damage Pyruvate Ammonia S alk(en)yl sulfenic acids Organosulphur compounds Allicin

  5. GARLIC & HEALTH Integration with development

  6. GARLIC & HEALTH • Radiolabelled intermediates identified using HPLC and HPLC-MS in comparison with chemically synthesised compounds (P3). • Differential display sequences and isolated and reanalysed by northern blots and in situ hybridisation, where possible, with other Allium sp. (P3) • First year harvests and analysis completed (P2). • Second year experimental material planted. (P2). • cDNA clones encoding alliinase isolated and sequenced (P2). Second Year Milestones

  7. GARLIC & HEALTH • DP.16: Pathway intermediates identified (P2, P3) • DP.17: First sulphur budget for garlic (P2) • DP. 18: Clones for alliinase Second Year Deliverables

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