The Effect of Resveratrol on Fat Storage in C. elegans

1. The Effect of Resveratrol on Fat Storage in C. elegans By Chris LoBello

2. Obesity http://www.cdc.gov/nccdphp/dnpa/obesity/trend/maps

3. Adipocytes http://whyfiles.org/276metabolic_syndrome/index.php?g=3.txt

4. http://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpghttp://www.nature.com/nri/journal/v6/n10/images/nri1937-f1.jpg

5. PPARg http://www.juliantrubin.com/encyclopedia/biochemistry/ppar.html

6. Chung and Kurtev- Sirt1 promotes fat mobilization in white adipocytes by repressing PPAR-g. Nature (2004) http://eresveratrol.com/content/00/01/43/84/95/userimages/ppar_gamma.pdf

7. Wood and Sinclair- Sirtuin activators mimic caloric restriction and delay ageing in metazoans. Nature (2004) Fei and Liu- Relevance of NAC-2, an Na+ coupled citrate transporter, to life span, body size and fat content in Caenorhabditis elegans. Biochemical Society (2004) http://www.nature.com/nature/journal/v430/n7000/fig_tab/nature02789_F2.html http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1224044&blobtype=pdf

8. Control Group: 0 microliters of resveratrol N= N2 Wild Type Strain Culture Group 1: 1 microliter of resveratrol N= N2 Wild Type Strain Culture Group 2: 10 microliters of resveratrol N= N2 Wild Type Strain Culture Group 3: 50 microliters of resveratrol N= N2 Wild Type Strain Culture Group 4: 100 microliters of resveratrol N= N2 Wild Type Strain Culture Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol Nile Red staining solution (.1microgram/ml) Done up to days 6 to 8 in the protocol Fluorescent intensity determined by using images taken from microscope to place in Photoshop to determine the strength of the fluorescence Statistical Analysis by ANOVA where p <.05 followed by Scheffe post hoc test Methodology The Effect of Resveratrol on Fat Storage in C. elegans

9. Budget

10. Do-Ability • C. elegans can be used as a valuable model organism for the study • Nile Red Staining will successfully help examine resveratrol’s effect on the C. elegans • Results of the staining can be quantified by using Photoshop

11. Purpose To determine the effects of varying amounts of resveratrol on the fat content of C. elegans

12. Hypothesis Null Hypothesis: There will be equal amounts of fat content in cells with resveratrol supplemented and without resveratrol supplemented Alternate Hypothesis: There will be less fat content in the cells supplemented with resveratrol, and that there will be an inverse relationship between resveratrol supplementation and fat content

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