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Resveratrol and Decreased Fat Deposition in C. elegans

Resveratrol and Decreased Fat Deposition in C. elegans. Alexandra ******- Period 3 Manhasset Science Research. Need. Obesity Trends* Among U.S. Adults 1990, 1998, 2007. (*BMI  30, or about 30 lbs. overweight for 5’4” person). 1998. 1990. 2007.

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Resveratrol and Decreased Fat Deposition in C. elegans

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  1. Resveratrol and Decreased Fat Deposition in C. elegans Alexandra ******- Period 3 Manhasset Science Research

  2. Need Obesity Trends* Among U.S. Adults1990, 1998, 2007 (*BMI 30, or about 30 lbs. overweight for 5’4” person) 1998 1990 2007 No Data <10% 10%–14% 15%–19% 20%–24% 25%–29% ≥30% Source: Center for Disease Control & Prevention [http://www.cdc.gov/nccdphp/dnpa/obesity/trend/maps/obesity_trends_2007.ppt#487,5,Slide 5]

  3. Knowledge Base Figure 1: Chemical structure of Nile Red (A) & Sudan Black (B) staining. Nile Red staining in wild-type and mutant C. elegans (C-E). Sudan Black staining in wild-type (F) & mutant (G) organisms. Source: (Ashrafi, 2007) [http://www.wormbook.org/chapters/www_obesity/obesity.html]

  4. Resveratrol • Chemical found in grapes; red wine • Popular dietary supplement- advertised as anti-aging substance • May explain “French paradox” Figure 2: chemical structure of resveratrol (C14H12O3) [http://www.3dchem.com/molecules.asp?ID=277]

  5. Literature Review Figure 2: RNAi was used to silence certain genes to determine which ones regulated fat in C. elegans (Ashrafi, et al, 2003) [chart made by author using materials from article]

  6. Literature Review • C. elegans in dauer need AMP-activated protein kinase (AMPK) to survive • Dauer- larval “hibernation” stage in which metabolic rate is slowed; fat deposits increase to sustain organism • AMPK- fat regulatory enzyme • aak2 mutant dauers lack AMPK destroy fat • (Roy, et al, 2009) Figure 3: The enzyme AMPK is necessary for C. elegans in dauer to survive and regulate fat deposition (Roy, et al, 2009) [chart created by author]

  7. Literature Review • Resveratrol improves health and survival of mice on a high-calorie diet • (Baur, et al, 2006) Figure 4: Resveratrol caused many positive changes in the overall health of mice similar to the effects of caloric restriction (Baur, et al, 2006) [chart made by author adapted from article]

  8. Literature Review Figure 5: In human preadipocytes (fat cell precursors), resveratrol in extremely high doses (3-5 g) caused positive changes in the cells. (Fischer-Posovsky, et al, 2008) [chart made by author]

  9. Purpose/Hypothesis • Purpose: to observe the effects of resveratrol on fat deposition in C. elegans and comparing it to caloric restriction • Null hypothesis: Resveratrol will cause no change in fat deposition in C. elegans • Alternate hypothesis: Resveratrol will cause a decrease in fat deposition and mimic the effects of caloric restriction

  10. Methodology

  11. Budget Total Price: $198.69

  12. Do-ability • Resveratrol studies have been done in previous years • C. elegans is a useful model organism that is easily obtained • Sudan Black staining and fluorescent microscope examination are possible

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