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Supplementary Fig. 1

A. 100. GRP78. 90. NS2. 80. vector. 70. 60. 50. RLA x 10 3. 40. 30. 20. 10. 0. ng. 70. 90. 50. 30. 10. 110. 1.75 x 10 5 Huh-7 cells. B. 3.5. 3.0. 2.5. Fold change (Dox+ / Dox-). 2.0. 1.5. 1.0. 0.5. 0. Days. 3. 4. 1. 2. Tet-on/NS2. Supplementary Fig. 1. A.

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Supplementary Fig. 1

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  1. A 100 GRP78 90 NS2 80 vector 70 60 50 RLA x 103 40 30 20 10 0 ng 70 90 50 30 10 110 1.75 x 105 Huh-7 cells B 3.5 3.0 2.5. Fold change (Dox+ / Dox-) 2.0 1.5 1.0 0.5 0 Days 3 4 1 2 Tet-on/NS2 Supplementary Fig. 1

  2. A IFN (+) IFN (-) - + IFN core actin B GRP78 GADD153 CMV ATF6 25 40 P < 0.0005 P < 0.0001 140 25 P < 0.0001 P < 0.0001 35 20 120 20 30 100 15 25 15 RLA x 104 80 RLA x 106 RLA x 106 RLA x 104 20 10 60 10 15 40 10 5 5 20 5 0 + - 0 + 0 0 - + - + - IFN IFN IFN IFN Supplementary Fig. 2

  3. A. 300 GRP78 promoter In Huh-7 150 * 35 30 RLA x 104 25 20 15 10 5 0 core null Tuni E1 NS2 E1-p7 E2 B. 75 70 GRP78 promoter In Huh-7.5 + SG 35 ** 30 RLA x103 25 20 15 10 5 0 E1-p7 core E1 E2 null Tuni NS2 -actin 28S RNA Supplementary Fig. 3

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