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Early Scientists’ Contributions (The dead guys you need to know!) And... The Cell Theory. Remember Water???. We already learned about several of water’s unique properties Polarity Adhesion Cohesion Surface Tension
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Early Scientists’ Contributions(The dead guys you need to know!)And... The Cell Theory
Remember Water??? • We already learned about several of water’s unique properties • Polarity • Adhesion • Cohesion • Surface Tension • Because of these properties, water “mounds-up” and is able to magnify whatever image is beneath it!
Let's Talk Microscopes... What does Magnify mean? - To increase the size What do you know about Microscopes? Do you know what a Compound Light Microscope is?
Anton van Leeuwonhoek Created the First Compound Microscope
Anton Van Leeuwenhoek • A tradesman from Holland. • Sold and Traded Drapery! • First to discover Bacteria • Considered the Father of Microbiology • What does Microbiology mean?_________________ • He created the first compound microscope which allowed people to see “organisms” never before seen! • He called them “animacules” • What is an organism??? ________________________________________
Electron Microscopes • Use electrons, not light, to view specimens • Advantages • Nanoscale imaging! (This means it can magnify the teeny-weeny stuff and show it on a computer screen for all to view!) • Disadvantages • Super expensive • Specimens must be viewed in a vacuum… which means, specimens must be DEAD!
Electron Microscopes… A dust mite A spider
Pure Cane Sugar Maggot Ant Bed Bug Virus
Cork Cells Blood Cells
Robert Hooke • Coined the term “cell” • Viewed cork cells under the lens...
What IS a cell, anyway? • A cell is the structural and functional unit of ALL organisms • It is the smallest unit of life • Often called the building block of life • Each cell is actually ALIVE! • Some organisms are unicellular • What does THAT mean???? _________________ • Some are multicellular • What does THAT mean???? _________________
Schleiden & Schwann Schleiden and Schwann came up with the theory that all plant life (Schleiden) and animal life (Schwann) are composed of cells, that these cells were made from cells and that they end up making more cells...
Cell Theory • The cell is the basic unit of structure & function of all living things. • All living things (organisms) are made of cells. • All cells come from pre-existing cells. • Francesco Redi started this line of thought! • Schleiden and Schwann helped it along... • Life doesn’t just “appear” out of thin air!
Two Main “Types” of Cells • Plant Cells • Animal Cells
Two Main “Groups” • There are two main “groups” of cells • - Prokaryote • (Pro=No…...No organelles!) • You’ll learn about those soon enough! • - Eukaryote • (You=Eu and Eu=Nu… You are a eukaryote because you are made of cells with organelles and a nucleus!)
Prokaryote Cells • No organelles. • No nucleus. • Always unicellular organisms. • Examples: bacteria
Eukaryotic Cells • Have organelles. • Have a nucleus. • Can be unicellular or multicellular. • Unicellular: • Yeast • Multicellular: • Plants and animals
Questions... • Who created the first compound microscope?__________________ • Who coined the term “cell”? _________________ • Who started the idea that cells (life) must come from existing cells (life)? ___________________________
More Questions... • What are the two “types” of cells? ____________________________________________________ • What are the two main “groups” of cells? ____________________________________________________ • What are WE? __________________________
Parts of the Microscope • There are a few parts of the Compound Light Microscope which you will NEED to know!
Eyepiece Ocular Lens Nosepiece Body Tube Objective Lenses (3) Arm Diaphragm Coarse adjustment knob Light Source Stage with Stage clips Base Fine adjustment knob 30
Nosepiece Body Tube Arm Light Source Base Stage with Stage clips
The total power of Magnification To calculate the power of magnification, multiply the power of the ocular lens by the power of the objective. What are the total powers of magnification for each of the objectives we have on our microscopes? Our objective lenses are 4x so... _______ 10x so... _________ 40x so... _________
4 – Slowly lower the cover slip, at an angle, on top of the drop. Cover Slip Lower slowly 5 – Place the slide on the stage and view it first with the red-banded objective. Once you see the image, you can rotate the nosepiece to view the slide with the different objectives. You do not need to use the stage clips when viewing wet-mount slides! How to make a wet-mount slide … 1 – Get a clean slide and coverslip. 2 – Place ONE drop of water in the middle of the slide. Don’t use too much or the water will run off the edge and make a mess! 3 – Place the edge of the cover slip on one side of the water drop.