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Cytometry T.Caspersson Stockholm 1934 - cytometry by light absorption - chromosome banding ( h2 )

Cellular Pathology R.Virchow , Berlin 1858 cellular pathology ( h1 ) K.von Rokitansky , Vienna macroscopic pathology. Cytometry T.Caspersson Stockholm 1934 - cytometry by light absorption - chromosome banding ( h2 ). Early developments In flow and image cytometry.

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Cytometry T.Caspersson Stockholm 1934 - cytometry by light absorption - chromosome banding ( h2 )

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  1. Cellular Pathology R.Virchow,Berlin 1858 cellular pathology(h1) K.von Rokitansky, Vienna macroscopic pathology Cytometry T.Caspersson Stockholm 1934 - cytometry by light absorption - chromosome banding (h2) Early developments In flow and image cytometry Flow Cytometry W.Coulter Hialeah, 1949/56 electronic blood cell counter (h3/h4) G.Wied Prague, Munich Berlin, Chicago 1946 TICAS image analysis 1968 (h5/6) L.KossVienna, NY 1952 histo-/cytopathology (h7/8) flow cytometry G.Ruhenstroth-Bauer, D.Zang Munich 1960 artifactual right skew of Coulter cell volume distribution curves (h13/17) M.Melamed NY 1960 cyto-/histopathology (h10) flow cytometry CC Lushbaugh Los Alamos 1962 biological right skew claim (h14/15) Z.Darzynkiewicz Warsaw, NY 1963 cell biology by flow cytometry (h11) L.Kamentsky NY 1965 - first flow cytometer by light absorption (h9) - computerized data evaluation&display B.Mayall LLL 1965 -CYDAC chromosome DNA analysis -editor Cytometry (h12) M.Fulwyler Los Alamos 1965 cell sorter (h16) M.van Dilla Los Alamos 1968 first fluorescence by flow cytometry (h19) W.Göhde Münster 1968 - first fluorescence flow cytometer Phywe-ICP11 - fluorescent DNA as tumor marker (EB) (h20-22) R.Thom, V.KachelMunich 1969 hydrodynamic focusing in electrical sizing eliminates artefactual right skew (h18) L.Herzenberg Stanford 1969 first fluorescence activated cell sorter (FACS) (h23) • G.ValetMunich/Martinsried 1972 • - cytometry bioinformatics 72/87 ) • predictive medicine by • cytomics 1997/2001 (h24-26) • - cell function in disease • - European background • - US background • innovations

  2. Foundation of Society for Analytical Cytology American Engineering Foundation conferences organizer: Sandford S.Cole (h27) (initial preference for image cytometry) BioPhysics/Ortho/Coulter/BD laser cytometers 1970 Davos (K.Preston) 1971 Henniker, NH (K.Preston) 1972 Saxtons River, VE (K.Preston) 1973 Asilomar, CA (M.Mendelsohn) 1975 Asilomar, CA (L.Wheeless) 1976 Pensacola, FL (P.Mullaney) 1978 Schloß Elmau/Mittenwald (T.Jovin) European conferences motivator: W.Göhde (mainly flow) Phywe ICP-11/22 mercury arc cytometer 1972 Heidelberg (M.Andreeff) 1973 Nijmegen (C.Haanen) 1975 Münster (W.Göhde) 1977 Vienna (D.Lutz) 1979 Voss (O.Laerum) 1980 Rome (F.Mauro) foundation of the Society for Analytical Cytology (SAC) & journal Cytometry at Schloß Elmau 1978 conference renamed: 1991:International Society for Analytical Cytology (ISAC) 2010:International Society for Advancement of Cytometry (ISAC)

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