1 / 8

Cell division – The Wakefield Lab

Cell division – The Wakefield Lab. What principles govern the formation of the mitotic spindle? How do they interact to ensure robustness and flexibility to the system? Why is the spindle the shape it is?. early embryos. Bioinformatics. System perturbation (genetics et al.).

remedios-graves
Download Presentation

Cell division – The Wakefield Lab

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Cell division – The Wakefield Lab What principles govern the formation of the mitotic spindle? How do they interact to ensure robustness and flexibility to the system? Why is the spindle the shape it is?

  2. early embryos Bioinformatics System perturbation (genetics et al.) Software engineering Mathematical modelling Proteomics Biochemistry qualitative synthetic quantitative analytical What is our approach? Observation (cell biology) • 13 rounds of mitosis within 3 hrs • Maternally produced protein • Syncytial and therefore synchronous • Can collect grammes within a week

  3. msd3 RNAi control msd1 RNAi What proteins that bind MTs are important for cell division? biochemically isolate Drosophila embryonic MAPs Identify via MS and categorise Characterise novel proteins live observation Correlation categorisation Hughes et al., (2008) PLoS Biol

  4. Applying MS data to protein interaction networks Generate extended network of putative MAPs Use MS data to produce a list of interspecies MAPs Rank using PCA/Random forest likelihood statistics Generate interesting hypotheses characterise candidates

  5. Purify via GFP-TRAP Metaphase Anaphase 171 63 49 65 13 7 What proteins are part of mitotic MAP complexes in vivo, and what PTMs are important for their function? metaphase chromosome condensation cytokinesis BOREALIN anaphase SURVIVIN INCENP AURORA B spindle organisation kinetochore:k-MT attachment Mass spec and analyse characterise candidates Identify phosphorylation and SUMOylation sites

  6. Wakefield Lab (Past) Julian Hughes Katherine Fisher (Present) Faisal Khan Pete Jones Dan Hayward Jack Chen Simon Li Acknowledgements Collaborations • Mass Spectrometry Nicole Zitzmann (Oxford) Hannah Florance (Exeter) • Bioinformatics Charlotte Deane (Oxford)

  7. all principles / processes What principles govern the formation of the mitotic spindle? How do they interact to ensure robustness and flexibility to the system? Why is the spindle the shape it is?

More Related