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Agilent Technologies

Agilent Technologies. Applications for Field Service Engineers. HPLC Applications Training. HPLC Applications Training. HPLC Applications Training. HPLC Applications Training. HPLC Applications Training. HPLC Applications Training. MOBILE PHASE CONSIDERATIONS Flammability Toxicity

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Agilent Technologies

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  1. Agilent Technologies Applications for Field Service Engineers

  2. HPLC Applications Training

  3. HPLC Applications Training

  4. HPLC Applications Training

  5. HPLC Applications Training

  6. HPLC Applications Training

  7. HPLC Applications Training • MOBILE PHASE CONSIDERATIONS • Flammability • Toxicity • Miscibility • Viscosity • Dissolved Salts • %Organic Solvent • pH (Acid/Alkali) • Carbon Dioxide • Corrosion

  8. HPLC Applications Training • Some Flammable Solvents Commonly Used • Acetonitrile • Methanol • Hexane, iso-Hexanes, Heptane, iso-Octane • Tetrahydrofuran • Toluene • Acetone • Ethyl Acetate • Isopropanol

  9. HPLC Applications Training • Some Toxic Solvents Commonly Used • Acetonitrile • Methanol • Hexane • Tetrahydrofuran • Toluene • Dichloromethane (Methylene Chloride) • Chloroform • Dimethyl Sulphoxide

  10. HPLC Applications Training

  11. HPLC Applications Training • HPLC SOLVENTS - UV CUTOFF • Burdick & Jackson solvents are arranged in order of increasing UV cutoff, • the wavelength at which the solvent absorbance in a 1 cm path length cell • is equal to 1 AU (absorbance unit) using water in the reference cell. • UV Cutoff (nm) • Acetonitrile UV 190 • Pentane 190 • Water 190 • Hexane UV 195 • Cyclopentane 198 • Cyclohexane 200 • Heptane 200 • Isopropyl Alcohol 205 • Methanol 205 • Ethyl Alcohol 210 • 2-Methoxyethanol 210 • Methyl t-Butyl Ether 210 • n-Propyl Alcohol 210 • Trifluoroacetic Acid 210 • Tetrahydrofuran UV 212 • n-Butyl Alcohol 215 • 1,4-Dioxane 215 • Ethyl Ether 215

  12. HPLC Applications Training

  13. HPLC Applications Training

  14. HPLC Applications Training

  15. HPLC Applications Training

  16. HPLC Applications Training

  17. HPLC Applications Training

  18. HPLC Applications Training

  19. HPLC Applications Training

  20. HPLC Applications Training

  21. HPLC Applications Training • HPLC Columns • Silica particles (1.5~100μm) • Polymer particles (3~50µm)

  22. HPLC Applications Training

  23. HPLC Applications Training

  24. HPLC Applications Training • SAMPLE PREPARATION • Filtration – Membrane Filters 0.2µm, 0.45µm (Cellulose Acetate, PVDF) • Centrifugation – Economical, less contamination, multiple processing

  25. HPLC Applications Training PS335 Photodiode Array Detector

  26. HPLC Applications Training PS335 Photodiode Array Detector

  27. PS335 Photodiode Array Detector HPLC Applications Training

  28. PS335 Photodiode Array Detector HPLC Applications Training

  29. PS335 Photodiode Array Detector HPLC Applications Training

  30. Agilent Technologies Applications for Field Service Engineers

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