Cell Transport: Active and Passive Movement

1. Warm-Up • What types of substances need to move into and out of cells? • What cellular organelle facilitates this movement?

2. Show Us What You Can Do! • With a partner…grab a mini white board (and a WB marker), and design an illustration that explains active and passive transport…you can’t use the picture that I drew…but you can be creative and or humorous  • Be prepared to explain what makes your illustration effective. You will do this with another pair of illustrators. • You should be prepared to offer and accept constructive criticism.

3. Lab: Cell Transport-Osmosis Pre-Lab Questions • Why is it necessary for materials to move into a cell? What types of substances are these? • Why is it necessary for materials to move out of a cell? What types of substances are these? • What is the limiting factor that is the reason that cells are not bigger than they are? • What is osmosis?

4. Some Important Pre-Lab Vocabulary • Hypertonic: the cell’s environment has a higher concentration of solutes (stuff) than inside the animal or plant cell. • Hypotonic: the cell’s environment has a lower concentration of solutes (stuff) than inside the animal or plant cell. • Isotonic: the cell’s environment has the same concentration of solutes (stuff) than inside the animal or plant cell.

5. What You’ll Be Doing • Over the course of the next several days, you will be immersing an egg in various solutions. • Prior to each immersion, you will hypothesize as to what will happen to the egg. • On each day that you perform this task, you will make detailed quantitative and qualitative observations about the egg. You will also record qualitative observations regarding the solution used.

6. For Example • On day one you will immerse the egg in 5% CH3COOH (Vinegar). • Your day 1 Hypothesis will be something like: I think that if the egg is immersed in 5% CH3COOH for one to two days, then …”. • You will record the circumference of the egg using the string provided • You will record the mass of the egg using the analytical balance. • You will record the color, texture, and any other observable traits of the egg.

7. You will record your observations regarding the solution as well (clear, red, cloudy, bubbles present, viscous (thick), sweet smelling, etc.).

8. 1 Egg White Vinegar (aka: acetic acid or ethanoic acid or…CH3COOH) Analytical Balance Ruler (metric) String H2O (water) 7. Corn Syrup 8. Food Coloring 9. Plastic Cup 10. NaCl (salt) *11. One liquid (safe) of your choice *If time permits Osmosis Lab:Materials

9. Procedure • With your partner, obtain one egg and one plastic cup. • Record your Day 1 hypothesis…you will be placing the egg in vinegar. • Use the analytical balance to mass your egg to the nearest 0.1 (1/10) gram. • Use the string and a metric ruler to measure the circumference of your egg in centimeters to the nearest 0.1 (1/10) cm. • Record any additional qualitative observations about your egg (color, texture, markings, etc.)

10. 7. Use a Sharpie to write the names of your group members on your plastic cup. 8. Gently place your egg in the plastic cup and carefully add the 5 % acetic acid solution (vinegar) until your egg is covered. 9. Observe for at least five (5) minutes and record any additional observations. 10. Place your cup in the designated area labeled with your class color.