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Microscopes

Learn about different types of microscopes, such as Light Scopes, Phase Contrast, Transmission Electron, and Scanning Electron, their unique features, advantages, disadvantages, and typical images captured with each. Discover how magnification, resolution, and contrast play crucial roles in microscopic observations.

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Microscopes

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  1. Microscopes Karen J. Dalton Community College of Baltimore County Catonsville Campus

  2. Qualities • Magnification • Make specimen appear larger • Increase magnification, decrease field of view • Resolution • Clarity and detail • Do 2 close objects appear as 2 or 1? • Contrast • Increase or decrease amount of light striking the specimen

  3. Light Scopes Compound Brightfield • Illumination: light • Magnification: glass lenses • Cost: cheap (under $2000) • Specimens • Can be living (external & internal movement) • Color (natural & stains) • Disadvantage • Resolution

  4. Cells infected with Rocky Mountain Spotted Fever Colon cancer Amoeba Onion Root Bacteria Penicillium Spores Blood Smear Typical Images

  5. Phase Contrast • Type of Light Microscope • Enhances contrasts of transparent and colorless objects by influencing the optical path of light • Light passing trough a transparent part of the specimen travels slower • Advantages • Better resolution and contrast of unstained specimens

  6. Hamster ovary cells Cell Cycle Stained bone Stained Epithelial Cells Fruit fly salivary gland chromosomes Typical Images

  7. Transmission Electron • Illumination: electrons • Magnification: magnets • Specimens • High resolution • Disadvantage • Cost: over $10,000 • Technical Training • Thin sections • Special stains • Only black and white • No movement

  8. Virus Bacteria Golgi Apparatus Sperm Liver Cell Typical Images

  9. Scanning Electron • Electron Microscope • Shows surface details

  10. Flea Head Tapeworm’s “Head” Pollen Leaf Cutter’s Head Typical Images Bird’s Feather

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