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Microscopes

Microscopes. Karen J. Dalton Community College of Baltimore County Catonsville Campus. Qualities. Magnification Make specimen appear larger Increase magnification, decrease field of view Resolution Clarity and detail Do 2 close objects appear as 2 or 1? Contrast

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Microscopes

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  1. Microscopes Karen J. Dalton Community College of Baltimore County Catonsville Campus

  2. Qualities • Magnification • Make specimen appear larger • Increase magnification, decrease field of view • Resolution • Clarity and detail • Do 2 close objects appear as 2 or 1? • Contrast • Increase or decrease amount of light striking the specimen

  3. Light Scopes Compound Brightfield • Illumination: light • Magnification: glass lenses • Cost: cheap (under $2000) • Specimens • Can be living (external & internal movement) • Color (natural & stains) • Disadvantage • Resolution

  4. Cells infected with Rocky Mountain Spotted Fever Colon cancer Amoeba Onion Root Bacteria Penicillium Spores Blood Smear Typical Images

  5. Phase Contrast • Type of Light Microscope • Enhances contrasts of transparent and colorless objects by influencing the optical path of light • Light passing trough a transparent part of the specimen travels slower • Advantages • Better resolution and contrast of unstained specimens

  6. Hamster ovary cells Cell Cycle Stained bone Stained Epithelial Cells Fruit fly salivary gland chromosomes Typical Images

  7. Transmission Electron • Illumination: electrons • Magnification: magnets • Specimens • High resolution • Disadvantage • Cost: over $10,000 • Technical Training • Thin sections • Special stains • Only black and white • No movement

  8. Virus Bacteria Golgi Apparatus Sperm Liver Cell Typical Images

  9. Scanning Electron • Electron Microscope • Shows surface details

  10. Flea Head Tapeworm’s “Head” Pollen Leaf Cutter’s Head Typical Images Bird’s Feather

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