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Biomanufacturing. At Mount Wachusett Community College. Number Suggested Course Order Cr BIO109 General Biology 4 BTC101 Introduction to Biotechnology 4 BTC102 Basic Solution & Media Preparation 3 ENG101 English Composition I 3 General Elective 3
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Biomanufacturing At Mount Wachusett Community College Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25
Number Suggested Course Order Cr • BIO109 General Biology 4 • BTC101 Introduction to Biotechnology 4 • BTC102 Basic Solution & Media Preparation 3 • ENG101 English Composition I 3 • General Elective 3 • CHE203 Introduction to Inorganic, Organic and Biochemistry 4 • Professional Elective 3/4 • CIS127 Computer Technologies 3 • ENG102 English Composition II 3 • MAT143 Probability and Statistics 3 • BIO160 Principles of Biochemistry 4 • BTC110 Biotechnology Experience: Concepts and Procedures 4 • HUM212 Medical Ethics 3 • PER126/130 Health, Fitness, & Wellness Elective 2/3 • BTC120 Biotechnology Experience: Biomanufacturing 4 • BTC200 Biotechnology Seminar 3 • BIO170 Cell Biology 4 • Social Science Elective 3 • Total: 60/62 credits Associate’s Degree Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25
4 credit class • Two lectures/week – 75 minutes each • One lab/week – 2 hours • OR • One class/lab per week – 4 ½ hours • Workforce Training Program • 3 days/week – 7 hours/day Class Format Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25
BTC120 Biomanufacturing Course Schedule Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25
Workforce Training Program Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25
Aseptic Technique Very important! Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25
CHO cells • Contain gene for tPA • Approximately 3 weeks • 100ml spinner flasks • 3L bioreactors Upstream Processing Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25
Centrifugation • Low pressure liquid chromatography • Ion exchange to purify tPA • ELISA • SDS-PAGE Downstream Processing Funded in full or in part by the U.S. Department of Labor Grant CB-17329-08-60-A-25