Iodimetric Titration of Vitamin C

1. By: Chris Lloyd and Katy Salter Iodimetric Titrationof Vitamin C

2. Background Information • Needed in the body to form collagen in the bones • Absorbed by the body but can not be stored • Lack of vitamin C results in Scurvy- disease that causes brown spots on the skins and gums will soften to the point of possible losing your teeth

3. Introduction • Tested levels of Vitamin C… • Time: Day After Purchase vs. 2 Weeks After • Temp: Room Temperature vs. Fridge • Storage: Open Container vs. Sealed

4. What We Did… • Made an Iodine solution • Titrated 25mL’s of orange juice sample until the endpoint • Used 1% starch solution as indicator

5. What We Used… • Tropicana Orange Juice • No Pulp, Non Concentrate • Vitamin C = 150% Daily Value • 60 mg Daily • One Bottle(355mL) = 90 mg • Theoretical grams of vitamin C per 25mL sample = 0.006338g or 6.338mg

8. Calculations • Actual Amount of Vitamin C per Sample: • Concentration of Standard=Concentration of Sample • (26.7/0.2515)=(6.27/x) • X=0.059g/L • Concentration of Sample x Volume of Sample=Actual grams in Sample • 0.059*0.025=0.00148g

9. Conclusion • Best Way to Store Orange Juice • Sealed in the fridge • Worst Way • Opened at room temperature • Two factors that will preserve Vitamin C • Sealed or in the fridge