Introduction to Cell & Molecular Biology, BIOL-2120

1. Introduction to Cell & Molecular Biology, BIOL-2120 • LABORATORY SESSIONS WILL MEET THIS WEEK • RECITATION SECTIONS WILL NOT MEET THIS WEEK (They begin next week)

2. Welcome to BIOL 2120: Introduction to Cell & Molecular Biology George Plopper, Ph.D. Department of Biology Course webpage: http://www.rpi.edu/~ploppg/biol_2120/biol_2120.html

3. BIOL 2120 BIOL 1010 (Sorry about that) Where does this class fit? Engineering (incl. BMED) Bio BCBP Bioinf Etc. Sciences Everything Else “Electives” Core Courses

4. What does this mean? • Taking this course represents a commitment to a scientific track: welcome to “being a biologist” (even if you are an engineering major). • From the syllabus: “You will be expected to think in this course, not just memorize.”

5. Grade history, by percentage

6. Bloom’s Taxonomy of Educational Objectives Bloom’s domains • cognitive (about knowing) • affective (about attitudes, feelings) • psychomotor (about doing) http://projects.coe.uga.edu/epltt/index.php?title=Bloom%27s_Taxonomy

7. Bloom’s Taxonomy Graduation Cognitive domain- A hierarchy of six levels: 6.create: makes judgments about the value of materials or methods; new knowledge 5.evaluate: can pull together many disorganized elements or parts so as to form a whole 4.analyze: can break down a communication into its constituent parts, revealingthe relationships among them 3.apply: all of levels 1 and 2, plus can take information of an abstract nature and use it in concrete situations 2.understand: can recall, but can do a little more (e.g. paraphrase, define, discuss to some extent) 1. remember: the recall of specific items This class Highschool

9. Forms of help • Course webpage: http://www.rpi.edu/~ploppg/biol_2120/biol_2120.html • Read the advice from previous years’ students • Resources: lecture outlines, Powerpoint files, recitation materials • Podcasts of lectures (from iTunes) • iClickerproblem solving in lecture

10. Forms of help • Graduate TAs: More than lab managers • Ask questions/discuss material during lab sections • BIOL2120rpi@gmail.com to contact the Head TA with any non-lab questions or concerns. • Office Hours: Reality check! Most exam questions arise from these discussions (I record them) • Office Hrs begin next week

11. Office Hrs Times/Locations Mon from 2:00-2:50pm: DCC 232 Tues from 3:00-3:50pm: DCC 232 Weds from 2:00-2:50pm: DCC 232 Thurs from 3:00-3:50pm: J-ROWL 1W01 Fri from 2:00-2:50pm: DCC 232

12. Forms of help Recitation sections: Small Group Learning • Led by former BIOL 2120 students who did very well and volunteered to help you this semester • What to expect: • Weekly quizzes (8 of them in the semester) • Informal discussion of lecture material • Relating lecture to real world examples: reading real researcharticles • Learn from experienced peers, at a student’s pace • What NOT to expect: • Perfect answers to every question you can come up with • Years of teaching experience • Endless entertainment Remember, the UTAs are one year ahead of most of you; they are not professors. Next year, you could be in their position.

13. Chapter 1 What Is a Cell? George Plopper

14. Welcome to Biology 2120 Introduction to Cell & Molecular Biology

15. Today’s Learning Outcomes: At the end of today’s class, you should be able to: • Draw a eukaryotic cell with at three least structural features other than the nucleus and plasma membrane. • List three properties of water that make it essential for life to exist on Earth • Explain, in writing, the differences between monosaccharides, disaccharides, and polysaccharides.

16. The Big Picture, Chapter 1 • Cells are NOT this: Nearly all cells are invisible to the naked eye Cells are composed of millions of molecules

17. Figure 01.01A: Examples of different cell types, including structures specific to each. Panel A, an epithelial cell.

18. Figure 01.01B: Panel B, a muscle cell.

19. Figure 01.01C: Panel C, a nerve cell.

20. Figure 01.01D: Panel D, Fibroblast

21. Figure 01.01E: Panel E, A plant cell

22. Figure 01.01F: Panel F, a prokaryotic cell.

23. The Big Picture, Chapter 1 • Cells are NOT this: Nearly all cells are invisible to the naked eye Cells are composed of millions of molecules Cells obey simple chemical rules

24. Figure 01.06A: Five unusual traits of water. Panel A, water is a liquid at room temperature. A. Hydrogen bonds form between partially charged atoms of opposite polarity. WATER IS SPECIAL

25. Figure 01.06B: Panel B, water is a polar molecule. B. CH4 and CO2 do not have partially charged atoms and therefore do not form hydrogen bonds.

26. Figure 01.06C: Panel C, the liquid phase of water is more dense than its solid phase (ice).

27. Figure 01.06D: Panel D, water has a very high specific heat and is a good termal insulator

28. Figure 01.06E: Panel E, water has a high heat of vaporization

29. CARBON IS SPECIAL Figure 01.08B: B. Model of a carbon atom bound to four other atoms.

30. Figure 01.09: The orientation of covalent bonds formed by carbon.

31. Table 01.T01: Common functional groups found in biological molecules. Note that dashed lines (-) indicate where these groups bond with other atoms/molecules.

32. Figure 01.10: Common types of lipids in cells. Common abbreviations of organic structures are shown.

33. iClicker time How does the valence number of an atom impact its function in living organisms? • It determines whether a molecule is hydrophobic or not. • It determines whether a molecule is charged or not. • It determines how many bonds it can form. • It determines the size of the atom. • It determines whether the molecule is alive or not.

34. iClicker time How does the valence number of an atom impact its function in living organisms? • It determines whether a molecule is hydrophobic or not. • It determines whether a molecule is charged or not. • It determines how many bonds it can form. • It determines the size of the atom. • It determines whether the molecule is alive or not.

35. SUGARS ARE VERY SPECIAL Figure 01.11: Common monosaccharides in cells. The carbons are numbered by convention with "primes" as shown.

36. Figure 01.12: Alpha and beta glycosidic bonds in common disaccharides.

37. Figure 01.13: Oligosaccharides and polysaccharides play many important roles in cells.

38. Today’s Learning Outcomes: At the end of today’s class, you should be able to: • Draw a eukaryotic cell with at three least structural features other than the nucleus and plasma membrane. • List three properties of water that make it essential for life to exist on Earth • Explain, in writing, the differences between monosaccharides, disaccharides, and polysaccharides.

39. For Next Lecture • Download lecture outline from course web page • Read Chapter 2 in Principles of Cell Biology • Learn how to draw the structure of ribose • Download recitation Module 1, packet #1, bring it with you to recitation section next week. Let me know of any technical difficulties.