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Michael Batista 1,2 Brian Carvalho 1,3 Department of Biological Engineering, MIT

Geometric Control of Cell Life and Death Christopher S. Chen, Milan Mrksich , Sui Huang, George M. Whitesides , and Donald E. Ingber Science, 1997. Michael Batista 1,2 Brian Carvalho 1,3 Department of Biological Engineering, MIT Department of Material Science Engineering, MIT

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Michael Batista 1,2 Brian Carvalho 1,3 Department of Biological Engineering, MIT

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  1. Geometric Control of Cell Life and DeathChristopher S. Chen, Milan Mrksich, Sui Huang, George M. Whitesides, and Donald E. IngberScience, 1997 Michael Batista1,2 Brian Carvalho1,3 Department of Biological Engineering, MIT Department of Material Science Engineering, MIT Department of Physics, MIT

  2. The Capabilities of Cancer Hanahanand Weinberg, Cell, Vol. 100, 57-70, 2000 SiemannDW., Vascular targeting agents. Horizons in CancerTherapeutics: From Beach to Bedside. 2002;3(2):4-15

  3. Apoptosis: ECM Adhesion vs. Cell Geometry Integrins: Transduce mechanical stimuli into/out of the cell Cell Shape: Implicated in triggering apoptosis independent of ECM Apoptosis 4.5µm Proliferation Differentiation Survival Apoptosis 100µm

  4. Cell Spreading and ECM Exposure Inhibits Apoptosis Nuclei Differences in cell curvature, bead engulfment, and ECM contact area confound the trend in B Restricting cells to flat pads eliminates these complications

  5. Apoptosis: Effect of Cell Surface Area Pad Size: Pad Spacing Holding ECM contact area constant does not change survival effect of increasing cell surface area.

  6. Apoptosis: Effect of Integrin Binding β-1 Ligand β-3 Ligand

  7. Conclusions

  8. Current Work: Mechanotransduction Mammary Epithelial LIF JAK/STAT3 MSCs RhoA ROCK Osteocytes Src ERK Apoptosis Differentiation Anti-Apoptosis

  9. Questions

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