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I. Basics

I. Basics. Electrophysiology is the study of cell electrical activity resulting from ion channel function. Electrolyte composition of the cell. Fundamental laws. Nernst's equation: E r = RT/F ln [C o /C i ] Driving force = E m - E r Ohm's law: V = R x I.

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I. Basics

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  1. I. Basics Electrophysiology is the study of cell electrical activity resulting from ion channel function.

  2. Electrolyte composition of the cell

  3. Fundamental laws • Nernst's equation: Er = RT/F ln [Co/Ci] • Driving force = Em - Er • Ohm's law: V = R x I

  4. A simplified view of cell-cell communication • Ligand-gated channels code for input • Voltage-gated channels code for output

  5. Baseline

  6.  Na leak

  7. GABAA IPSPs

  8. GABAB IPSP

  9. Baseline

  10. Subthreshold AMPA EPSP

  11. Threshold AMPA EPSP

  12. NMDA EPSP

  13. NMDA + GABAA PSPs

  14. II. Methods

  15. Selective antagonism of GABA responses in the cuneate nucleus of the anesthetized rat

  16. Configurations of the patch-clamp technique

  17. Configurations of the patch-clamp technique

  18. Patch-clamp dates

  19. The voltage-clamp technique

  20. III. From channels to behavior The thalamocortical augmented response (AR)

  21. Circuits involved in the AR

  22. Cellular mechanism of the AR

  23. Behavioral component of the AR

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