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SLIDE SHOW I Wild-type mechanism

SLIDE SHOW I Wild-type mechanism. +. -. -. -. A. In the ground state the permease is protonated. IX. R 302. E 325. X. H 322. VIII. K 319. H +. E 269. +. D 240. R. +. C. 144. VII. 148. -. V. E 126. IV. +. -. -. -. -. +. B. Substrate binds from the outside. IX.

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SLIDE SHOW I Wild-type mechanism

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  1. SLIDE SHOW I Wild-type mechanism

  2. + - - - A. In the ground state the permease is protonated IX R302 E325 X H322 VIII K319 H+ E269 + D240 R + C 144 VII 148 - V E126 IV

  3. + - - - - + B. Substrate binds from the outside IX R302 E325 X H322 VIII K319 H+o E269 + D240 R C 144 So VII 148 V E126 IV

  4. C. Binding of substrate causes a conformational change disrupting the interaction of Glu269 and His322 and …. + - - - IX R302 E325 X H322 VIII K319 H+ E269 + D240 R + C 144 So VII 148 - V E126 IV

  5. D. ... causes substrate to become accessible to the internal surface, and the proton moves to His322/Glu325. + - - - + IX R302 H+ E325 VIII H322 X K319 E269 D240 R + C 144 VII Si 148 - V E126 IV

  6. E. Rotation of Helix X continues towards the hydrophobic phase of the membrane, + - - - + IX R302 H+ E325 VIII H322 X K319 E269 D240 R + C 144 Si VII 148 - V E126 IV

  7. + - - - + F. raising the pKa of Glu325. H+ IX R302 VIII E325 H322 K319 X E269 D240 R + C 144 Si VII 148 - V E126 IV

  8. + - - - - + + G. Substrate dissociates to the inside, H+ IX R302 VIII E325 H322 K319 X E269 Si D240 R C Si 144 VII 148 V E126 IV

  9. + - - - - + + H. And Glu325 re-juxtaposes with Arg302. H+ IX R302 VIII E325 H322 K319 X E269 D240 R C 144 VII 148 V E126 IV

  10. + - - - - + I. The proton is released to the inside, IX R302 E325 X H+i H322 VIII K319 E269 + D240 R C 144 VII 148 V E126 IV

  11. + - - - J. the permease relaxes into the ground state and becomes protonated from the outside. IX R302 E325 X H322 VIII K319 H+o E269 + D240 R + C 144 VII 148 - V E126 IV

  12. MTS-1-MTS MTS-3-MTS o-PDM p-PDM no linker _ _ _ _ + + TDG + + N7C5 C5 K. Crosslinking between Asp240 (helix VII) and Lys319 (helix X): Effect of ligand binding MTS-1-MTS: 3 Å MTS-3-MTS: 5 Å; flexible o-PDM: 6 Å; rigid p-PDM: 10 Å; rigid

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