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LB145 Sec 001-004

LB145 Sec 001-004. Today’s Outline. Tutorial Reminder Homework #1: Results Homework #2: Assigned Organelles Nucleus, RER, Golgi Apparatus, Cytoskeleton, SER, Ribosomes, Lysosomes, Peroxisomes, Vacuoles, Mitochondria, Chloroplasts Match’Em Exercise. Last chance to register clickers!.

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LB145 Sec 001-004

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  1. LB145Sec 001-004

  2. Today’s Outline • Tutorial Reminder • Homework #1: Results • Homework #2: Assigned • Organelles • Nucleus, RER, Golgi Apparatus, Cytoskeleton, SER, Ribosomes, Lysosomes, Peroxisomes, Vacuoles, Mitochondria, Chloroplasts • Match’Em Exercise

  3. Last chance to register clickers! • Register your clicker by the end of this week. Exam Review • Tuesday Review Session (Trivia Game). • Special office hours: Wednesday morning.

  4. Homework 1: • Average = 6.24/7.00 (Excellent!) • Grades are on ANGEL along with my comments. • Next time – if you do not submit it to TurnItIn, it will not receive a score. • Next time – academic integrity… • You were shown in class a good example of what a violation of academic integrity looks like. Come see me if you have more questions or if you would like to see the de-identified example again.

  5. This is an example of Academic Dishonesty 1. Academic dishonesty includes (but is not limited to): 1.3. plagiarism: representing the words or ideas of another as one's own in any academic exercise (e.g., papers, computer programming assignments, class presentation, or laboratory reports); 1.4. submitting the same paper, or substantially similar papers, to meet the requirements of more than one course without the consent of all instructors concerned; 2. Instructors in Lyman Briggs are expected to take appropriate action in all instances of academic dishonesty…. In cases of academic dishonesty, an instructor may take action consistent with his/her syllabus, including giving a failing grade on the assignment, giving a lower or failing grade for the course, and/or recommending additional disciplinary action. [See website addendum under Academic Honesty]

  6. Homework #2: Northern European Folklore: Wehedem Kind, das beimKuß auf die Stirnsalzigschmekt, eristverhext und muss bald sterbe! "Woe to the child which when kissed on the forehead tastes salty. He is bewitched and soon will die!”

  7. Eukaryotes: Organelles to Live By • Nucleus • Ribosomes • Rough Endoplasmic Reticulum (RER) • Smooth Endoplasmic Reticulum (SER) • Golgi Apparatus • Peroxisomes • Lysosomes • Vacuoles • Mitochondria • Chloroplasts • Cytoskeleton

  8. The Nucleus

  9. Ribosomes

  10. Ribosome and rRNA • rRNA is made in the nucleus. • Small and Large Subunit proteins are transported INTO the nucleus. Where the ribosome is assembled. (below: protein in purple,rRNA in orange and yellow) Prokaryotes have THREE strands of rRNA. Eukaryotes have FOUR strands of rRNA. 5S 23S 5S 5.8S 28S 16S 18S What does this have to do with the lab?

  11. Keeping RNAs Straight • mRNA • MESSANGER RNA • Transcribed from DNA in nucleus. • Transported out into cytosol. • Interacts with ribosome where translation takes place and a protein is formed. • rRNA • RIBOSOMAL RNA • Transcribed from DNA in nucleus • Binds to large and small ribosomal subunits in nucleus – acts as structural support. • Exits nucleus (bound to ribosomal subunits) via nuclear pore. • tRNA • TRANSFER RNA • Transcribed from DNA in nucleus • Exits into cytosol via nuclear pore • Picks up a specific amino acid (covalent bond) in cytosol and transfers it to growing polypeptide chain.

  12. Some proteins are made in the cytoplasm (cytosol) on “free” ribosomes http://members.aol.com/SHinrichs9/abiopb/flow.jpg

  13. The Rough ER

  14. Some proteins are made on “bound” ribosomes They enter the endomembrane system Campbell 8e, Fig. 17.21

  15. The Golgi Apparatus

  16. The Endomembrane System: Manufacturing and Shipping Proteins

  17. Insulin is Secreted via the Endomembrane System Campbell 8e, Fig. 6.16

  18. Cytoskeleton • Microtubules (green) • Microfilaments (red) • Nucleus (blue)

  19. Molecular Components of the Cytoskeleton

  20. A Motor Protein Generates Motile Forces (Class of 1984)

  21. The Smooth ER

  22. Smooth ER • Synthesize fatty acids and phospholipids. • Breaks down toxins/poisonous lipids. • Reservoir Ca+ ions.

  23. Peroxisomes

  24. Peroxisomes • Center of oxidation reactions. • E.g. glyoxisomes oxidizing fat to store it as usable energy. • Break down very long fatty acid chains to shorter carbon chains. • These shorter chains are eventually shipped to mitochondrion and used to produce ATP.

  25. Lysosomes

  26. Lysosomes • Digestion packets full of enzymes. • Found in animal cells. • Fuse with vesicles or old organelles. • Often a very acidic environment. • Low pH. • Lots and lots of hydrogen ions.

  27. Vacuole

  28. Vacuoles • In plants and fungi. • Sometimes used to aid in digestion. • Store water and help cell maintain volume.

  29. Mitochondria

  30. Mitochondria – Generates ATP from Glucose Campbell 8e, Fig. 6.17

  31. Chloroplasts

  32. Chloroplasts – Generate G3P (glucose precursor) from sunlight and CO2 Campbell 8e, Fig. 6.18

  33. Activity – Cells and Organelles

  34. Coming Up Next Week: • Homework 2 is due on Tuesday • The Case of Cystic Fibrosis • Upload to TurnItIn (no identifying marks) • Bring a copy to class (last four #s of PID) • Tuesday: • Evaluate HW • Review for Exam 1. • Exam #1 is on the 24th.

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