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LB145 Sec 001-004

LB145 Sec 001-004. Happy Valentine’s Day!. Today’s Announcements. StudyNotes5 AND Homework4 are due on Tuesday Feb 19 th . **Download HW VERSION 2.0** Field Trip sign up – started! Glycolysis and the Krebs Cycle. Oxidative Phosphorylation, the ETC. Track the Carbons and Phosphates:

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LB145 Sec 001-004

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  1. LB145Sec 001-004 Happy Valentine’s Day!

  2. Today’s Announcements • StudyNotes5 AND Homework4 are due on Tuesday Feb 19th. • **Download HW VERSION 2.0** • Field Trip sign up – started! • Glycolysis and the Krebs Cycle. • Oxidative Phosphorylation, the ETC.

  3. Track the Carbons and Phosphates: Glucose + ATP  Fructose-P + ADP Fructose-P + ATP  P-Fructose-P P-Fructose-P  P-CCC + P-CCC P-CCC + P-CCC + 2NAD+ + 2Pi P-CCC-P + P-CCC-P + 2NADH P-CCC-P + P-CCC-P + 2ADP  P-CCC + P-CCC + 2ATP P-CCC + P-CCC + 2ADP  Pyruvate + Pyruvate + 2ATP Glycolysis: What do you need to know?

  4. Methods of Producing ATP GLYCOLYSIS & CITRIC ACID CYCLE Substrate-level phosphorylation occurs when ATP is produced by the enzyme-catalyzed transfer of a phosphate group from an intermediate substrate to ADP.

  5. Campbell: Fig. 9-6-3 Electrons carried via NADH and FADH2 Electrons carried via NADH Oxidative phosphorylation: electron transport and chemiosmosis Citric acid cycle Glycolysis Pyruvate Glucose Mitochondrion Cytosol ATP ATP ATP Substrate-level phosphorylation Substrate-level phosphorylation Oxidative phosphorylation

  6. Fig. 9-10 CYTOSOL MITOCHONDRION NAD+ NADH + H+ 2 1 3 Acetyl CoA Coenzyme A Pyruvate CO2 Transport protein

  7. Fig. 9-11 Pyruvate CO2 NAD+ CoA NADH + H+ Acetyl CoA CoA CoA Citric acid cycle 2 CO2 FADH2 3 NAD+ NADH 3 FAD + 3 H+ ADP + P i ATP

  8. Bitter Melon Selling my Body to Science Consume a cup of liquid glucose. Consume capsules that contain bitter melon OR placebo. Lie still on a bed with a dome over my head and allow breath to be analyzed for an hour. QUESTIONS: Q1: On a molecular level, what is happening if bitter melon aids weight loss? Q2: On a molecular level, what is happening if bitter melon does not aid weight loss? Q3: Why were they interested in the air I exhaled? Q4: What do you think might happen to excess glucose in your body?

  9. Glycolysis is a core metabolic pathway!

  10. Campbell 8e Fig. 9.16

  11. How does this compare to light reactions?

  12. ETC: The main pieces • Complex I: NADH dehydrogenase • removes e- from NADH • pumps 4 H+ into inter-membrane space • Complex II: succinate dehydrogenase • moves e- from FADH2 to Q • Complex III: Cytochrome bc1 • transfers e- from Q to Cytc • pumps 2 H+ into intermembrane space • Complex IV: cytochrome c oxidase • transfers e- from Cytc to O2 • pumps 2H+ into intermembrane space • Q: ubiquinone, pumps 2 H+ into intermembrane space • Cytc: cytochrome c • e- shuttles

  13. Disrupting cellular respiration:What happens if…..?

  14. Disrupting cellular respiration:What happens if…..? • Cyanide • Binds to Cytochromeoxidase (Complex IV) • Immediate Effect? • Ultimate Effect?

  15. Disrupting cellular respiration:What happens if…..? • Rotenone • Binds to ubiquinone binding site of Complex I • Immediate Effect? • Ultimate Effect?

  16. Disrupting cellular respiration:What happens if…..? • Dinitrophenol • Protonophore (makes membrane completely permeable to H+ ions) • Immediate Effect? • Ultimate Effect?

  17. Anaerobic Respiration

  18. Anaerobic Respiration (from wikipedia)

  19. Homework Problem: Question: The Glucose Problem In the molecular process known as cellular respiration, how many ATP molecules can be made from the electron transport chain after one molecule of glucose is catabolized in glycolysis, pyruvate processing and the citric acid cycle. Assume that 1 ATP is produced when 3.5 protons pass through ATP synthase. **What pieces of information do I need to know to solve this problem?

  20. Problem solving…

  21. Problem solving… What would be the implications if a mitochondria was missing Complex II?

  22. Problem solving… If Complex I was missing, what would net more ATP: Krebs Cycle OR ETC?

  23. Problem solving… Can the ETC make energy (ATP) when we are not breathing? Why or why not?

  24. Problem solving… Assume there was an unlimited supply of readily available NADH and an unlimited supply of readily available photons. If you had to choose one of the below two processes to make ATP, which would you choose (and why!)

  25. For TUESDAY: • SN5 and HW4 (Version 2.0) • Honors Option Students – SIGN UP FOR THE FIELD TRIP (and show me the money)

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