'Cloning vector' presentation slideshows

Molecular cloning

Lecture 6. Molecular cloning. With thanks to David Tscharke @ RSB. Lecture overview. AKA gene cloning or DNA cloning The generation of identical copies of a piece of DNA Propagated in bacteria that originate from a single cell Was the first recombinant DNA technology

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8.0 RECOMBINANT DNA TECHNOLOGY BY : NUR HIDAYAH MUHAMAD SALEH

8.0 RECOMBINANT DNA TECHNOLOGY BY : NUR HIDAYAH MUHAMAD SALEH. 8.1 RECOMBINANT DNA TECHNOLOGY 8.2 METHODS IN GENE CLONING 8.3 APPLICATION OF RECOMBINANT DNA TECHNOLOGY 8.4 ISOLATING DNA- Experiments. Alba turns green when exposed to blue light. 8.1 RECOMBINANT DNA TECHNOLOGY.

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DNA Technology Genetic Engineering

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???? (gene manipulation) ???? (molecular cloning) ????????????????????

???? (gene manipulation) ???? (molecular cloning) ???????????????????? ????????????????????????????????????????????????????????????????????????? (1 plasmid, 1 cell) ??????????????????????

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Cloning into Plasmids

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Cloning Genes

Cloning Genes. Gene cloning: amplifying a specific piece of DNA via a bacteria cell Cloning vector: a replicating DNA molecule attached with a foreign DNA fragment to be introduced into a cell Has features that make it easier to insert DNA and select for presence of vector in cell.

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5 Stages involved in GE

5 Stages involved in GE. Isolation Cutting Ligation and Insertion Transformation Cloning & Expression. Donor DNA. Position of gene of interest. Genetic probe. 1. Isolation. (a) Isolation of a specific gene from donor e.g. human. Cells broken open. Genetic probe added.

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