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Can You See Strawberries’ DNA?. Let’s Explore! What does DNA look like ?. Make a model !. Fruit Snacks = Base Pairs Guanine – Cytosine Adenine – Thymine Rules = G/C always go together A/T always go together Pick any colors to represent them
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Can You See Strawberries’ DNA?
Let’s Explore! What does DNA look like? • Make a model! • Fruit Snacks = Base Pairs • Guanine – Cytosine • Adenine – Thymine • Rules = • G/C always go together • A/T always go together • Pick any colors to represent them • Stay consistent if you pick yellow/red for GC, stay with it. • Toothpicks = Rungs on the Ladder. Carefully poke the toothpicks through your base pairs. • Twizzlers = Sugar phosphate backbone. Attach to ladder rungs. http://www.youtube.com/watch?v=m-dQdq0LO2E
Now! Back to the Question! Can you see Strawberry DNA? Let’s try! First – We’ll watch a video of some cool scientists who will show us a method for extracting DNA from a strawberry. http://youtu.be/hOpu4iN5Bh4
Do you think you can do it? I know you can! Here’s what you need! 1 ziploc bag ½ cup of water strawberries frozen or very ripe fresh 2 teaspoons of dish detergent 1 teaspoon of salt 2 plastic cups coffee filter ice cold rubbing alcohol (90%) stirring apparatus (popsicle stick or Qtip)
Team Up! Get with a partner. Send a partner to collect your materials.
First Add 2-3 strawberries to your bag. Zip and mush completely.
Second Combine: ½ cup water 2 teaspoons dish soap ½ teaspoon salt stir well
Third Add: Soap solution to strawberry bag. Mix, but not enough to make a lot of bubbles.
Fourth Filter: Pour the strawberry and soap solution into the cup THROUGH the coffee filter. Be careful!
Fifth Pour: Pour an equal amount of the very cold alcohol into the cup of strawberry/soap solution. (This is really DNA in solution).
Sixth Observe! Strawberry DNA will appear! You did it! You did it! Use the Q-Tip to place it under the microscope if you like. Or you can spread it on the black paper and look at it through the lense. What do you see?