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In The Name Of God

Antigen/Antibody Reaction. Primary reaction :Immunoassay :Like Elisa , RIA , IFASecondary reaction : Lattice formation like Agglutination , Hemagglutination Complement Fixation, Neutralization , Precipitation : soluble antibody reacts with soluble antigenTertiary reaction : Detected by a

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In The Name Of God

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    1. In The Name Of God Precipitation

    2. Antigen/Antibody Reaction Primary reaction : Immunoassay :Like Elisa , RIA , IFA Secondary reaction : Lattice formation like Agglutination , Hemagglutination Complement Fixation, Neutralization , Precipitation : soluble antibody reacts with soluble antigen Tertiary reaction : Detected by affect on tissues or cells.

    3. Secondary Phenomenon

    4. precipitation : proteins Several types: Protein precipitation SRID DID ImmunoElectherophoresis: Counter current IEP Rocket IEP

    5. SRID plate preparation: (Agarose ?+ BUFFER ( Ab (Anti IgG ) Punch Sampling Incubation in RT for 24h measuring of diameter making of curve Calculation of sample concentration

    6. SRID Plate

    9. Single Radial Immuno diffusion : In SRID : 34 GAM = Is useful for measuring of IgG , IgA, IgM,C3, C4 Gel is mixed with Ab Ag is in punch In Single Radial Diffusion: one component is fixed (e.g. antigen-in this case it is IgG) As the Ag diffuses into the gel it reacts with the Ab and when the equivalence point is reached a ring of precipitation is formed as illustrated in Figure The diameter of the ring is proportional to the concentration of Ag .

    11. (Double Immuno Diffusion)= DID or Ouchterlony Immunodiffusion Qualitative 2 Diffusion Application in research laboratory Is not as a routin method now

    14. Ouchterlony - Identity

    15. Ouchterlony – Partial Identity

    16. Ouchterlony Non-Identity

    17. Turbidimetery measuring the amount of light Passing Through the solution.

    18. Nephelometery Measures SCATTERED light

    19. Immunoelectrophoresis

    20. Thanks For your Attention

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