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Supplement fig. 1 Expression of Activin A and ACVR in HPDLC-3O and -3Q. ( A ). ( B ). ACVR. Supplemental Fig.1 Expression of Activin A and ACVRs in HPDLC-3O and -3Q. Immunolocalization of Activin A was examined using cells
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Supplement fig. 1 Expression of Activin A and ACVR in HPDLC-3O and -3Q (A) (B) ACVR Supplemental Fig.1 Expression of Activin A and ACVRs in HPDLC-3O and -3Q. Immunolocalizationof Activin A was examined using cells derived from molar teeth of two individual patients: HPDLC-3O, derived from a premolar of a 20-year-old female (upper), and -3Q, derived from a premolar of a 21-year-old female (bottom). No staining was observed in control samples (without primary antibodies). Nuclei were stained with hematoxylin. Experiments were performed in duplicate. Bars = 100 mm. (B) Semi-quantitative RT-PCR analysis revealed the expression of genes encoding inhibinbaand four ACVRs (Ia, Ib, IIa, IIb) in HPDLC-3O (left) and -3Q (right). These results support the findings presented in Fig. 1. anti-Activin A control IaIbIIaIIb