Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS

1. Extraction of Gasoline from Soil Samples Using Supercritical Fluid Extraction and GC-MS By: Tim Abelland Zach Bensley

2. Purpose • To create a method to extract gasoline from soil samples.

3. Importance • Test contamination of soil after an oil spill. • To see if clean up from an oil spill was successful.

4. Procedure • Sample Preparation (SFE): • Approximately 1.00 g of celite spiked with 0.50 mL of gasoline. • 3 samples were prepared. • Placed spiked sample in SFE column. • 50 mL of chloroform in beaker in an ice bath to capture the extracted gasoline. • Extracted each sample for 30 minutes.

5. Procedure • Sample Preparation (GC-MS): • Created Standard with 0.50 mL of gasoline and 100 mL chloroform. • Each sample mixture was diluted to 100 mL with chloroform. • Ran standard and 3 sample on GC-MS.

6. Data • Standard

7. Data • Celite Trial 1

8. Data • Celite Trial 2

9. Data • Celite Trial 3

10. Procedure • Sample Preparation (SFE): • Obtained soil from outside. • Approximately 5.00 g of soil with 0.50 mL gasoline. • 3 samples were prepared. • Placed spiked soil samples in SFE column. • Placed 50.0 mL chloroform in a beaker in an ice bath. • Ran each sample for 30 minutes.

11. Procedure • Sample Preparation (GC-MS): • Created Standard with 0.50 mL of gasoline and 100 mL chloroform. • Each sample mixture was diluted to 100 mL with chloroform. • Ran standard and 3 sample on GC-MS.

12. Data • Soil Sample Trial 1

13. Data • Soil Sample Trial 2

14. Data • Soil Sample Trial 3

15. Error • Making soil sample more fine. • Longer run time on SFE for soil samples. • Gasoline and chloroform mixture sat for 6 days.

16. Conclusion • Gasoline can be extracted using SFE and condensed in chloroform. • More work with the method of extraction with soil samples.

17. Future Work • Test with different solvents. • Extract non-spiked soil samples.