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Department of Medical Laboratory Sciences Tarleton State University Dale Telgenhoff Sally Lewis

BLAST (Basic Laboratory Skills Testing): Evolution of a Practical Laboratory Skills Foundation to Support Undergraduate Research. Department of Medical Laboratory Sciences Tarleton State University Dale Telgenhoff Sally Lewis. Rational for Development of BLAST. Mission of MLS

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Department of Medical Laboratory Sciences Tarleton State University Dale Telgenhoff Sally Lewis

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  1. BLAST (Basic Laboratory Skills Testing): Evolution of a Practical Laboratory Skills Foundation to Support Undergraduate Research Department of Medical Laboratory Sciences Tarleton State University Dale Telgenhoff Sally Lewis

  2. Rational for Development of BLAST • Mission of MLS • History of research in MLS • Past UG research experiences • Goals • Prepare UG for research prior to experience • Preparation for graduate school

  3. Creation Phase • Identify medium for project • Define outcomes • Identify Faculty/Project Coordinator • Communicate desired project goals and outcomes to Project Coordinator and MLS faculty • Departmental support

  4. You want me to do what??????

  5. Defining Outcomes • Students • Faculty

  6. Defining Outcomes

  7. Calming the Beast

  8. Initiation Phase: Boot Camp I Identification of Basic Skills Separation of “Basic” and “Advanced” Review of Skills Previously Learned Assessment of Mastery

  9. What is a “Basic Skill”?

  10. Skill Selection Basic Skills Advanced Skills Phlebotomy Specimen collection Cell counting Instrument operation pH adjustment Spectrophotometry Osmometry Molecular techniques • Hand washing • Disinfecting countertops • Cleaning up biohazard spill • Removal of contaminated gloves, clothing • Selection of proper waste receptacle • Microscopy • Koehler Illumination • Pipetting • Monitoring temperatures • Dilutions • Lab Math • Gravimetric analysis • Centrifugation

  11. Skill Categories Biosafety Pipetting Microscopy Dilutions and Solutions • Handwashing • PPE • Biohazard clean-up • Waste management • Procedure • Selection • Micropipettes • Troubleshooting • Components • Optimal imaging • Koehler • Modifications • Glassware selection • Concentration mathematics • Gravimetric analysis • Centrifugation

  12. Instructional Phase: Boot Camp I

  13. Format • Four 2-hour sessions • Hands-on laboratory instruction • Time dedicated for practice • Facilities available for additional practice

  14. Instructional Session Example • Handwashing – GloGermHandwash • Spill clean-up • Segregation of waste

  15. Assessment: Boot Camp I

  16. Assessment Stations • One-on-one performance of skill in front of faculty • Four stations, rotation of students • Pass/fail system – three attempts

  17. Remediation • Six fails – all in pipetting skills • One-on-one instruction/ troubleshooting • Improved technique, passed skills test

  18. Here we Go Again: Boot Camp II Increased emphasis on pipetting skills Incorporated BLAST into grade for Introductory Course Reviewed feedback from faculty, students

  19. Undergraduate Research • Basic lab skills required for advanced molecular techniques • Overall improvement of skills – little need for remedial teaching • More time spent on research development and implementation

  20. Clinical Outreach • Discuss basic lab skills with clinical instructors • Review of techniques currently used • Skills improved – still need emphasis on pipetting skills Information provided by Virginia Reyes, M.Ed., MT(ASCP)SBB

  21. Boot Camps III +: What we have Learned…. Some skills replaced, substituted Continued strong emphasis on pipetting skills, assessment Graded component of Introductory course Fear – spread by word of mouth – increased practice!

  22. Questions?

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