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The Effect of Wind on the Rate of Transpiration . Nicole Chernavsky Nadia Zivkov Cassandra Zhi. Research Question. How does wind strength* affect the rate of transpiration? *D ifferent fan distances of wind (40 cm, 70 cm, 100 cm, 130 cm, 160 cm). Why is this important?.
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The Effect of Wind on the Rate of Transpiration Nicole ChernavskyNadia ZivkovCassandra Zhi
Research Question • How does wind strength* affect the rate of transpiration? *Different fan distances of wind (40 cm, 70 cm, 100 cm, 130 cm, 160 cm)
Why is this important? • Certain species of plants require different amounts of water due to different rates of transpiration, which vary depending on the wind level of the environment • Plants in more windy environments will require more water • When there is too much wind, the stomata may even close • Too expensive to water certain plants • For farmers, it is too expensive to farm certain crops because of the extensive amount of water needed to counter the excessive amount of transpiration caused by a windy environment • Therefore, the wind level should be taken into consideration when farmers choose which crops to plant at different times of the year in order to prevent excess transpiration or closure of the stomata
Variables • Independent variable: Distance of plant cuttings from fan (cm) (40, 70, 100, 130, 160 cm) • Dependent variable: Barometric pressure (mm Hg) created by transpiration of plant cutting
Materials • Materials: • PASPORT Xplorer GLX • 3 PASPORT Barometer pressure sensors • 3 PASPORT extension cables • Large tub of water • 6 utility clamps • 3 plastic tubing clamps • 3 plastic tubings • 3 ring stands • 1 razor blade • 3 95-watt lamps • 1 metric ruler • 1 large fan • 3 plant cuttings • Flashdrive GLX Sensor
Method • Obtain 3 plant cuttings Jar with water
Method cont. • Keep the clipped ends of the plant cuttings submerged in water • Cut plant at 45° angle and immediately place into open end of plastic tubing
Method cont. • Afterward, clamp plant end with plastic tubing clamp Keep thumb over top to maintain constant pressure! Pressurized tube with water
Method cont. Plant cutting • Set up structure as shown in the photo (make sure to keep the barometer level with the plant cutting) Fan Barometer Clamps
Method cont. • Do 3 trials with 5 levels of independent variable • The distance of the fan from the plant cuttings is changed: 40 cm, 70 cm, 100 cm, 130 cm, and 160 cm from the plant cuttings • For each variable, run trial for 10 minutes, letting GLX sensor measure every 10 seconds
Method cont. • Record data, process, and analyze data
Results-Tables Data Table #1: Average Rate of Transpiration (in mmHg/10seconds) for Different Fan Distances from the Plant Cutting for Trials A,B, and C
Results- Graphs Graph #1: Fan Distance From Plant Cutting (in cm. ) vs. the Average Rate of transpiration (in mmHg/10seconds)
Results-Photos 95 Watt Lamps Fan Plant cuttings GLX sensor Clamp
Conclusion • Too much wind is not good for transpiration • Stomata closes and causes little to no transpiration • Stomata close/wind strong=sensor reads positive pressure • For the plant cuttings placed 40 cm from the wind source, the plants transpired slowly or not at all • The rate of transpiration, in this case, was actually positive 0.02 mmHg/10sec • Plants experiencing this much wind would thus starve • For the plant cuttings that were 70 cm and 100 cm from the wind source, the data recorded was not viable to reach conclusions • An air bubble formed and the sensor could not measure pressure changes
Conclusion cont. • For the 130 cm, data showed that there was a transpiration rate of -0.05535 mmHg/10 sec • This showed that at this wind level, transpiration rates were relatively high • For the 160 cm, data showed that there was a transpiration rate of -0.02385 mmHg/10 sec • This showed that at this wind level, transpiration rates were not as high as the 130 cm rate • This is because there is less wind evaporating the water on the surface of the leaf, implying lower transpiration rates • Overall, stronger wind leads to more transpiration, but too much wind causes no transpiration because stomata close
Evaluation • Some errors include: • After the 100 cm trial, it was apparent that the plant cuttings were no longer submerged in the water • The leaf cutting in the middle seemed to have more wind directed toward it because it was placed directly in front of the fan as opposed to the other two leaf cuttings which were placed toward the sides of the fan • During some of the trials, objects blocked parts of the fan (i.e. the power outlet blocked part of the fan during the 100 cm trial)