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Zebrafish in Genome Research

Zebrafish in Genome Research. Can you spot the difference?. What is a zebrafish ?. Danio rerio Small freshwater fish from South Asia. 4 cm long when fully grown. Common aquarium fish. Very easy to look after. What is a model organism?.

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Zebrafish in Genome Research

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  1. Zebrafish in Genome Research Can you spot the difference?

  2. What is a zebrafish? • Daniorerio • Small freshwater fish from South Asia. • 4 cm long when fully grown. • Common aquarium fish. • Very easy to look after.

  3. What is a model organism? • Non-human species widely studied to understand human disease. • Model organisms are used when experimentation using humans is unfeasible or unethical. Can you think of a model organism?

  4. Types of model organism

  5. Why use zebrafish? • Small size. • All major organs present within5 days post fertilisation. • Short generation time (3-4 months). • Produces 300-400 eggs every2 weeks. • Translucent embryos. • Lots of genome resources available.

  6. THE ZebrafishemBRYO brain ear muscle block segments swim bladder eye heart notochord ~3.5 mm

  7. Modelling human conditions • Zebrafish mutants have been produced to modelhuman diseases such as: • Alzheimer's disease • Congenital heart disease • Polycystic kidney disease • Duchenne muscular dystrophy • Malignant melanoma • Leukaemia

  8. Forward screening for mutants P x F1 x F2 x F3

  9. Reverse screening for mutants Potential human disease gene Exciting gene expression pattern Gene of interest Potential new player in developmental pathway Gene knockout Phenotype analysis

  10. The Activity Identify differences between the wild type zebrafish and mutant zebrafish • A glossary is provided to help you with scientific terms

  11. Flash cards & worksheets

  12. Answers

  13. Answers – Image 1 • What’s the difference? Embryo B has no eye

  14. Answers – Image 2 • What’s the difference? Fish B is a lighter, golden colour compared to fish A.

  15. Answers – Image 3 • What’s the difference? The body of fry B is curved. If you look closely you’ll also see that its mouth is open. This is because it is unable to fully close its mouth as its muscles are too weak.

  16. Answers – Image 4 • What’s the difference? The zebrafish embryos in picture B look paler and are not stained red.

  17. Answers – Image 5 • What’s the difference? There are bright green blobs in picture B.

  18. Answers – Image 6 • What’s the difference? Embryo A has more blue dots than embryo B. The blue dots are stained neutrophils moving towards a wound on the zebrafish fin.

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