1 / 41

Aspergillus: Diagnostic Methods and Classification Overview

Explore the classification, diagnostic techniques, and characteristics of Aspergillus fungi, including species like A. fumigatus and A. niger. Learn about indirect and direct diagnostic methods for accurate identification. Contact Dr. Patricio Godoy Martínez for more information.

smoorhead
Download Presentation

Aspergillus: Diagnostic Methods and Classification Overview

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Aspergillus Classic Diagnostic Dr. Patricio Godoy Martínez Universidad Austral de Chile pgodoymartinez@gmail.com

  2. Aspergillus • Ubiquitous, commonly ocurring in soil, water, and decaying vegatation. • Reservoirs in hospitals; unfiltered air, ventilation system, carpeting, food, water distribution system, etc. • Cause invasive and allergic disease in humans and other animals. • Cause plant and food spoilage and produce mycotoxins.

  3. Classification of Aspergillus. ~260 especies, 38 have caused disease Balajee & Marr,2006. Future Microbiology, 1:435-445.

  4. Sexual stages associated with the genus Aspergillus Geiser 2009. Medical Mycology, 47:S21-S26

  5. Aspergillus Mycology Diagnostics Indirect Direct

  6. Aspergillus Direct Methods Colony Microscopic examination Detection of Ag and A.N Coloration Histopathology

  7. hyphaes hyalines septate. Direct microscopic examination Vitale R

  8. Histopathology: H&E Vitale R

  9. Histopathology: Grocott Vitale R

  10. Identification

  11. Macro-microscopic observation of colonies

  12. Morphological character

  13. Morphological character COLUMNAR RADIATE Josep Guarro

  14. Aspergillus fumigatus Colonies black blue-green, consisting of a dense felt of conidiophores. www. pgodoy. com

  15. Aspergillus fumigatus Conidial heads columnar. Conidiogenous cells uniseriate. Conidiophore stipes smooth-walled often green in the upper part. Conidia verrucose. www.pgodoy.com www.aspergillus.man.ac.uk

  16. Neosartorya • Anamorphs closely related to A.fumigatus. • Teleomorphs only differentiated by subtle differences in ascospores ornamentation. • Thermophilic.

  17. Neosartorya • N. fischeri • N. pseudofischeri • N. hiratsukae

  18. Neosartorya • Osteomyelitis • Keratitis • Endocarditis • Peritonitis • Cerebral infection • Pulmonary infection • Disseminated infection

  19. Neosartorya pseudofischeri

  20. Neosartorya hiratsukae Josep Guarro

  21. N. hiratsukae N. pseudofischeri Josep Guarro

  22. Josep Guarro

  23. Neosartorya • Underdiagnosed (regarded as contaminants in the lab) • Difficult to identify at species level. • Antifungal susceptibility similar to A. fumigatus ? • Little experience in the treatment. Amphotericin B and itraconazole Josep Guarro

  24. Aspergillus niger Colonies black, consisting of a dense felt of conidiophores. www. pgodoy. com

  25. Aspergillus niger Conidial heads radiate. Vesicles subspherical, conidiogenous cells biseriate. Conidia brown, ornamented. www.aspergillus.man.ac.uk

  26. Aspergillus flavus Colonies yellowish-green, consisting of a dense felt of conidiophores. www.aspergillus.man.ac.uk www.pgodoy.com

  27. Aspergillus flavus Conidial heads radiate. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia echinulate. Sclerotia may be present. www.aspergillus.man.ac.uk

  28. R. Salim & R. Runco Aspergillus parasiticus Colonies yellowish-brown, consisting of a dense felt of conidiophores. conidiophores of varying lengths, wall hyaline, thick, with ornamentation equinulada, spherical vesicles, uni or biseriate (24-25 m), completely covering the fiálides gallbladder. Conidia globose or subglobosos warted (2.5-3 m) with thin walls (20, 21).

  29. Aspergillus tamarii Conidial heads compact and spherical or loosely radiate. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia echinulate. www.aspergillus.man.ac.uk

  30. Aspergillus orizae Conidial heads radiate to loosely columnar. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia smooth-walled to roughened. www.aspergillus.man.ac.uk

  31. Aspergillus terreus Colonies yellowish-brown to cinnamon-brown, consisting of a dense felt of conidiophores. www.aspergillus.man.ac.uk

  32. Aspergillus terreus Conidial heads densely columnar. Conidiogenous cells biseriate. Conidiophore stipes smooth-walled hyaline. Conidia smooth walled, striate hyaline. www.aspergillus.man.ac.uk

  33. Aspergillus nidulans Colonies growing rapidly, green, cream-buff or honey-yellow, reverse dark purple. Vitale R

  34. Aspergillus nidulans Conidial heads short, columnar. Conidiogenous cells biseriate. Conidiophore stipes brownish. Conidia spherical, rugulose, subhyaline, green. Vitale R

  35. Aspergillus clavatus Colonies growing rapidly, bluish-green, consisting of a dense felt of conidiophores.

  36. Aspergillus clavatus Conidial heads radiate, later splitting into several columns. Conidiogenous cells uniseriate. Conidiophore stipes smooth. Vesicle clavate. Conidia smooth-walled hyaline. www.pgodoy.com

  37. INFOCUS 2010 - CHILE

More Related