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Aspergillus Classic Diagnostic

Aspergillus Classic Diagnostic. Dr. Patricio Godoy Martínez Universidad Austral de Chile pgodoymartinez@gmail.com. Aspergillus. Ubiquitous, commonly ocurring in soil, water, and decaying vegatation.

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Aspergillus Classic Diagnostic

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  1. Aspergillus Classic Diagnostic Dr. Patricio Godoy Martínez Universidad Austral de Chile pgodoymartinez@gmail.com

  2. Aspergillus • Ubiquitous, commonly ocurring in soil, water, and decaying vegatation. • Reservoirs in hospitals; unfiltered air, ventilation system, carpeting, food, water distribution system, etc. • Cause invasive and allergic disease in humans and other animals. • Cause plant and food spoilage and produce mycotoxins.

  3. Classification of Aspergillus. ~260 especies, 38 have caused disease Balajee & Marr,2006. Future Microbiology, 1:435-445.

  4. Sexual stages associated with the genus Aspergillus Geiser 2009. Medical Mycology, 47:S21-S26

  5. Aspergillus Mycology Diagnostics Indirect Direct

  6. Aspergillus Direct Methods Colony Microscopic examination Detection of Ag and A.N Coloration Histopathology

  7. hyphaes hyalines septate. Direct microscopic examination Vitale R

  8. Histopathology: H&E Vitale R

  9. Histopathology: Grocott Vitale R

  10. Identification

  11. Macro-microscopic observation of colonies

  12. Morphological character

  13. Morphological character COLUMNAR RADIATE Josep Guarro

  14. Aspergillus fumigatus Colonies black blue-green, consisting of a dense felt of conidiophores. www. pgodoy. com

  15. Aspergillus fumigatus Conidial heads columnar. Conidiogenous cells uniseriate. Conidiophore stipes smooth-walled often green in the upper part. Conidia verrucose. www.pgodoy.com www.aspergillus.man.ac.uk

  16. Neosartorya • Anamorphs closely related to A.fumigatus. • Teleomorphs only differentiated by subtle differences in ascospores ornamentation. • Thermophilic.

  17. Neosartorya • N. fischeri • N. pseudofischeri • N. hiratsukae

  18. Neosartorya • Osteomyelitis • Keratitis • Endocarditis • Peritonitis • Cerebral infection • Pulmonary infection • Disseminated infection

  19. Neosartorya pseudofischeri

  20. Neosartorya hiratsukae Josep Guarro

  21. N. hiratsukae N. pseudofischeri Josep Guarro

  22. Josep Guarro

  23. Neosartorya • Underdiagnosed (regarded as contaminants in the lab) • Difficult to identify at species level. • Antifungal susceptibility similar to A. fumigatus ? • Little experience in the treatment. Amphotericin B and itraconazole Josep Guarro

  24. Aspergillus niger Colonies black, consisting of a dense felt of conidiophores. www. pgodoy. com

  25. Aspergillus niger Conidial heads radiate. Vesicles subspherical, conidiogenous cells biseriate. Conidia brown, ornamented. www.aspergillus.man.ac.uk

  26. Aspergillus flavus Colonies yellowish-green, consisting of a dense felt of conidiophores. www.aspergillus.man.ac.uk www.pgodoy.com

  27. Aspergillus flavus Conidial heads radiate. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia echinulate. Sclerotia may be present. www.aspergillus.man.ac.uk

  28. R. Salim & R. Runco Aspergillus parasiticus Colonies yellowish-brown, consisting of a dense felt of conidiophores. conidiophores of varying lengths, wall hyaline, thick, with ornamentation equinulada, spherical vesicles, uni or biseriate (24-25 m), completely covering the fiálides gallbladder. Conidia globose or subglobosos warted (2.5-3 m) with thin walls (20, 21).

  29. Aspergillus tamarii Conidial heads compact and spherical or loosely radiate. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia echinulate. www.aspergillus.man.ac.uk

  30. Aspergillus orizae Conidial heads radiate to loosely columnar. Conidiogenous cells uni and biseriate. Conidiophore stipes rough-walled hyaline. Conidia smooth-walled to roughened. www.aspergillus.man.ac.uk

  31. Aspergillus terreus Colonies yellowish-brown to cinnamon-brown, consisting of a dense felt of conidiophores. www.aspergillus.man.ac.uk

  32. Aspergillus terreus Conidial heads densely columnar. Conidiogenous cells biseriate. Conidiophore stipes smooth-walled hyaline. Conidia smooth walled, striate hyaline. www.aspergillus.man.ac.uk

  33. Aspergillus nidulans Colonies growing rapidly, green, cream-buff or honey-yellow, reverse dark purple. Vitale R

  34. Aspergillus nidulans Conidial heads short, columnar. Conidiogenous cells biseriate. Conidiophore stipes brownish. Conidia spherical, rugulose, subhyaline, green. Vitale R

  35. Aspergillus clavatus Colonies growing rapidly, bluish-green, consisting of a dense felt of conidiophores.

  36. Aspergillus clavatus Conidial heads radiate, later splitting into several columns. Conidiogenous cells uniseriate. Conidiophore stipes smooth. Vesicle clavate. Conidia smooth-walled hyaline. www.pgodoy.com

  37. INFOCUS 2010 - CHILE

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