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QUIZ Lab 2

QUIZ Lab 2. 10 points, 10 minutes. Ag-Ab Reaction. Assist.Prof.Suwin Wongwajana,M.D.,M.Sc. Dept.of Microbiology, Fac of Medicine, KKU. Write your name , student code , lab gr. Date on the top of the paper. 8 items , 10 marks, 10 min.

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QUIZ Lab 2

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  1. QUIZ Lab 2 10 points, 10 minutes Ag-Ab Reaction Assist.Prof.Suwin Wongwajana,M.D.,M.Sc. Dept.of Microbiology, Fac of Medicine, KKU.

  2. Write yourname,student code,lab gr.Dateon the top of the paper 8 items, 10 marks, 10 min.

  3. Patient serum was diluted inserial 4-folded manner. After adding the Agand let the reaction occurred. The precipitate was found until tube No.3. Theantibody titer=?(Ifdilution in tube No.1 = 1:4 ) 1:4 1:16 1:24 1:64 1: 256

  4. 2.About single radial immunodiffusion (SRID), Which sentence is correct? It is an agglutination technique. It uses electricity in the test. It is sensitive thanELISA. It is a quantitative technique. All of the above.

  5. 3.The steps inimmunoelectrophoresis (IEP) test included: a. let Ag react with Ab b. puncture the gel, add Ag into the wellc. run the electrophoresis d. add Ab into the trough Which sequence is correct? a. abcd b. bcda c. cdba d. dabc e. No correct answer

  6. 4.The results of immunodiffusion was shown, Which sentence is correct? Ab Ab Ag1 Ag3 Ag2 Ag1andAg2 arenon-identity Ag1andAg2 arepartial identity Ag1andAg3 arepartial identity Ag2 andAg3 areidentity No correct answer

  7. 5.About blood grouping test, If you have blood gr.O, Which sentence is correct? RBC have Ag A andAg B. In serumhave anti-A, Noanti-B In serumhave anti-B, Noanti-A. RBC have no Ag A andno Ag B. No correct answer.

  8. 6.AboutELISA technique, Which sentence is incorrect? Useenzyme as the labelling agent. Can detect both Ag or Ab. Must add the substrate then observing the color changes. The intensity of the color changed is direct correlated with the concentration of the unknown. Fluorescent microscope was required in order to assess the result.

  9. 7.Which sentence is incorrect? Gr Ocan give the RBC to the Gr.B Gr Ocan give the RBC to the Gr.AB Gr Acan give the RBC to the Gr.AB The lowest percentage of Thai Bl.Gr.is AB The highest percentage of Thai Bl.Gr.is B

  10. 8. 1 ml 1 ml 0.5 ml 0.1 ml 3 99 4.5 9.9 1:4 ................ .............. ................ Dilution Final Dilution 1:80 ................ .............. ................

  11. Answer No. 1-7, 1 mark each No. 8, 0.5 mark each Total 10 marks

  12. Patient serum was diluted inserial 4-folded manner. After adding the Agand let the reaction occurred. The precipitate was found until tube No.3. Theantibody titer=?(Ifdilution in tube No.1 = 1:4 ) • 1:4 • 1:16 • 1:24 • 1:64 • 1: 256 = 4 X 4 X 4

  13. 2.About single radial immunodiffusion (SRID), Which sentence is correct? • It is an agglutination technique. • It uses electricity in the test. • It is sensitive thanELISA. • It is a quantitative technique. • All of the above.

  14. 3.The steps inimmunoelectrophoresis (IEP) test included: a. let Ag react with Ab b. puncture the gel, add Ag into the wellc. run the electrophoresis d. add Ab into the trough Which sequence is correct? a. abcd b. bcda c. cdba d. dabc e. No correct answer

  15. 4.The results of immunodiffusion was shown, Which sentence is correct? Ab Ab Ag1 Ag3 Ag2 • Ag1andAg2 arenon-identity • Ag1andAg2 arepartial identity • Ag1andAg3 arepartial identity • Ag2 andAg3 areidentity • No correct answer

  16. 5.About blood grouping test, If you have blood gr.O, Which sentence is correct? • RBC have Ag A andAg B. • In serumhave anti-A, Noanti-B • In serumhave anti-B, Noanti-A. • RBC have no Ag A andno Ag B. • No correct answer.

  17. 6.AboutELISA technique, Which sentence is incorrect? • Useenzyme as the labelling agent. • Can detect both Ag or Ab. • Must add the substrate then observing the color changes. • The intensity of the color changed is direct correlated with the concentration of the unknown. • Fluorescent microscope was required in order to assess the result.

  18. 7.Which sentence is incorrect? • Gr Ocan give the RBC to the Gr.B • Gr Ocan give the RBC to the Gr.AB • Gr Acan give the RBC to the Gr.AB • The lowest percentage of Thai Bl.Gr.is AB • The highest percentage of Thai Bl.Gr.is B = O

  19. 8. 1 ml 1 ml 0.5 ml 0.1 ml 3 99 4.5 9.9 1:4 1:100 1:10 1:100 Dilution Final Dilution 1:80 1:8,000 1:80,000 1:8,000,000

  20. No. 1-7, 1 mark each No. 8, 0.5 mark each Total 10 marks Write the-name of the checker-Total scoreSend them back to teacher

  21. Good Luck • Good Luck

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