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PERFUSI & EUTHANASIA

PERFUSI & EUTHANASIA. Perfusi Adalah tehnik persiapan preparat dengan mengeluarkan darah dari tubuh sebanyak-banyaknya , kemudian digantikan dengan cairan latex. Langkah-Langkah Lakukan anesthesi total pada kambing dengan menggunakan chloralhydrat melalui vena Jugularis

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PERFUSI & EUTHANASIA

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  1. PERFUSI & EUTHANASIA

  2. PerfusiAdalahtehnikpersiapanpreparatdenganmengeluarkandarahdaritubuhsebanyak-banyaknya, kemudiandigantikandengancairan latex

  3. Langkah-Langkah • Lakukananesthesi total padakambingdenganmenggunakanchloralhydratmelalui vena Jugularis • Tunggusampaikesadaranhilang, lalulakukaninsisipadabagianleherpadalokasi a. carotiscommunis • Klemartericarotiscommunispadaduatempat, dankemudianinsisiarteritersebutditengah-tengahantarakeduaarteriklem • Masukkanselangpadaartericarotiscommunisbeberapa cm menujuarahjantungdanikatselangpadaarterimenggunakanbenang (arteriklemjangandilepasterlebihdahulu)

  4. Setelahselangterikatkuatpadaarteri, lepaskansalahsatuklemarteridimanaselangdimasukkan, sehinggadarahdariarteritersebutakankeluardantampungpadatasplastiksampaidarahberhentimengalir Setelahdarahberhentimengalir, suntikkancairan latex (kira-kirasejumlahdarah yang keluar) sampaicairan latex terlihatpada vena yang paling jauhdarijantung

  5. Setelah latex terdeteksipada vena yang paling jauhdarijantung, suntikkancairan formalin keseluruhtubuh (setiap 5 cm persegisatuspuit 20 ml ) agar cadaver tidakmembusuk. Masukkan cadaver kedalambakberisicairan formalin

  6. Perlengkapan Setiappraktikumwajibmembawa: Jas Lab. Dissecting set Gloves Masker

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