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The Nucleus

The Nucleus. Text and image sources are included using the notes function of the .ppt file. Interphase Nuclear Dynamics. Time Lapse Video. DIC Brightfield Euchromatin. EM thin section view. Chromatin organization.

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The Nucleus

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  1. The Nucleus Text and image sources are included using the notes function of the .ppt file

  2. Interphase Nuclear Dynamics Time Lapse Video DIC Brightfield Euchromatin

  3. EM thin section view

  4. Chromatin organization early replication -> late replication chromosomal organization one cell cycle later lamin B and late replicated DNA lamin B and early replicated DNA

  5. 40x condensation Packing it all in 146 bp; 6x condensation

  6. The HistoneCode

  7. Anchoring the genome LAPs (Lamin associated proteins) anchor the lamina to the inner envelope

  8. Freeze etch view of the lamina

  9. The nuclear matrix

  10. Diversity of nuclear shapes

  11. Eosinophil nuclei are constricted

  12. Chromosomal domains

  13. Nuclear compartments

  14. Promyelocytic leukemia (PML) nuclear bodies Video

  15. Total RNA production (fluorouridine pulse)

  16. Nucleolar Substructure Yeast cell Mammalian cell

  17. Polymerase I transcribes rDNA in nucleolar fibrillar centers 500 nm

  18. Gene Expression Factories Euchromatin (acH3),DNA (DAPI),Spliceosomal protein SC-35 Animated reconstruction

  19. Spliceosomes contain poly-A RNA

  20. Nuclear speckle dynamics 3 Video Sequences Time lapse sequences show SC-35 protein over 30 min periods

  21. NuclearmRNA localizations Collagen A1 mRNA mRNA (red) Gene (green) Epstein-Barr Viral mRNAs

  22. Nuclear traffic

  23. Freeze Fracture Negative Stain Visualizing Nuclear Pores Thin Section CryoEM Tomographic Reconstruction

  24. Annulate lamellae store pores 400 nm

  25. Nuclear pores In vitro regulation by Ca++

  26. Transport of nucleoplasmin-coated gold particles

  27. It takes two (or three) to tango Digitonin-permeabilized cells incubated with NLS*BSA*TRITC -cytosol +cytosol +cytosol, GTP

  28. Nup358 Nup214 Nup153 Nup 98 Nup 62 NLS*BSA + cytosolic factorsidentifies nucleoporins on a Western-style blot

  29. Nuclear Transport Exportin NES Exportin NES Ran*GTP Ran*GTP Ran-GAP Ran-GEF Ran*GDP Ran*GDP Importin Importin NLS NLS Cytoplasmic compartment Nuclear compartment

  30. FG affinity transport

  31. Follow the FG-repeatsFollow the FG-repeatsFollow, follow, follow, follow Animation

  32. The nucleus - not just a place to find DNA anymore

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