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Varian 940-LC Analytical to Prep HPLC

Varian 940-LC Analytical to Prep HPLC. Varian 940-LC Silver Bullets. The 940-LC one instrument – many uses: High performance automated Analytical HPLC High performance semi-preparative HPLC High performance prep LC. Varian 940-LC Silver Bullets. Flexible and capable

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Varian 940-LC Analytical to Prep HPLC

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  1. Varian 940-LC Analytical to Prep HPLC

  2. Varian 940-LC Silver Bullets • The 940-LC one instrument – many uses: • High performance automated Analytical HPLC • High performance semi-preparative HPLC • High performance prep LC

  3. Varian 940-LC Silver Bullets • Flexible and capable • Wide flow rate from 0.1 ml/min to 200 ml/min • Dual path-length flowcells for extended Abs • One Injector, analytical or prep volumes (5ul to 4.5ml) • Superb fraction collecting • Galaxie real-time peak detection • Practical collect and inject • Much Easier interface, it does what you tell it! • Cleaner UI

  4. Goals of the 940-LC Analytical to Prep • Aim • Become market leader in this $180M segment • Double market share • Market • Continue to focus on Pharma / BioPharma • Academic • Strategy • Deliver innovation in hardware, consumables, software and services

  5. Varian 940-LC Instrument Overview • Factory tested, pre-plumbed instrument: • Plug and play operation • High performance semi-preparative HPLC • Gradient Analytical HPLC • Autoprep System • Choice of UV or PDA detector • Dual path flow cell for unmatched range and scalability • No compromise performance for prep and analytical – switch with a key stroke – no plumbing or hardware changes needed! • The most flexible analytical to prep HPLC available

  6. Varian 940-LC Instrument Overview • Scale-up Module for automated methods transfer from analytical to semi-prep, or switching from Analytical to Prep work • Versatile and robust solvent-delivery system • New loopless autosampler – wide volume range • NEW Fraction collection with • Complete range of collection modes such as slope, threshold and time slicing • Wide range of vessels, including autosampler rack • Comprehensive range of columns and consumables • More productivity via GalaxieTM software • Valve automation for genuine unattended operation • NEW direct control interface

  7. Varian 940-LC Versatility and Performance • Easy to change pump heads • Versatile solvent delivery systems • Powerful pumps allow 4000psi at 25mL/min operation for fast chromatography • Ideal for 4.6mm and 1” column • Able to use smaller 3 & 5 um particles columns at elevated pressures • Improves productivity • Save solvents and waste costs • Short run times save time • High resolution separations = high purity 1ml/min Analytical 21ml/min Prep

  8. Varian 940-LC Solvent Delivery System • Same range of accessories as PS210/218 • 5ml Head to 200ml Head • Seal wash option available • Easily interchangeable pressure modules thru removable side panel. • Easy access to mixer • Self maintenance and versatility features have been completely retained with no compromises – integrated systems are notoriously difficult to maintain so this is Unique

  9. Varian 940-LC Autosampler • Prep version of the 920-LC Sampler • 5ul to 4.5ml Injection range • No necessity to combine a Manual injector • No need to dedicate injector to prep or analytical • One Sampler does all! • Loopless Design • Variable injection volume • Needle in path Design • No carryover

  10. Varian 940-LC UV-Vis Detector • High Sensitivity for Analytical applications • Extended absorbance – desensitised, for prep applications • All in one detector • Wide l range 190 to 900 nm • Low noise of < 5 x 10-6 Au • Self –aligned D2 lamp • Consistent performance • Easy to change over without tools • Front panel access • Easy to maintain

  11. Information Rich Diode Array Detection • 1024 photodiodes full coverage from 190 – 950 nm • Full l spectra collection • Enhanced peak purity parameter • Automatic peak identification with customised libraries • Greater confidence on compound identification • Choice of flowcells • Dual path length scale-up • Dual path length Prep • Front panel access • Lamps and flowcells • D2 and Vis lamps self aligned • Easily user maintained

  12. Varian 940-LC PDA Detectors • PDA allows enhanced peak purity determination • Galaxie uses whole spectrum across peak to determine purity

  13. Varian 940-LC Dual Pathlength Flowcell • Absorbance Detector • Extended absorbance range without re-plumbing (<70 AU) • NEW 4mm x 0.25 mm • 4mm x 0.15 SuperPrep • Low dispersion • UV/Vis Detector • PDA Detector • Low noise ~ +10 mAU • Low Drift ~ 1 mAU/hr

  14. Competitors Flowcells

  15. Varian 380-LC and 385-LC ELSD • Universal HPLC detector • Compatible with gradients • More sensitive than RI • NEW Glass Nebuliser for Improved Reproducibility • 2 to 3 Times More Sensitive • 40Hz Data Rate for Fast LC • Complete Galaxie compatibility • Program changes thru a run

  16. Varian 440-LC Fraction Collector • Full software control with foolproof collection by slope, threshold, volume or timeslice • Known fluid path for accurate fraction collection • Software display of collected fractions and source injections • Reliable operation • Compatible with a wide range of containers • Belart and other optional racks • Microvial to 50ml tube capacity • Autosampler rack compatibility provides practical collect and re-inject capabilities

  17. Varian 440-LC Fraction Collector • We chose not to collect Peak 3 • Peaks 1,2,4,5,6,7 collect with exceptional accuracy • Excellent algorithm, and simple delay volume calculation • Slope/Threshold based on real numbers!!

  18. Varian 440-LC Fraction Collector • Fraction Log shows peak location using colour coded rack assignment

  19. Optimized flow paths for automated scale-up Separate optimised flow paths for Prep and Analytical columns Solvent selection valves for 4 solvents (2 per pump) Integrated priming valves for easy solvent changes Integrated electrically actuated valves for analytical and prep Injections Clear cover to protect operators against leaks Varian 445-LC Scale-up Module

  20. Varian 940-LC Software Usability • Software designed for Prep users • Enhanced manual control • One page status and chromatogram • Real time fraction collection display • Advanced peak sensing for better fraction collection • Purity displays for absorbance detection – standard! • Documentation and audit trails for traceability and regulatory needs

  21. Varian 940-LC Integrated HPLC • Special features of the Varian 940-LC • Leak detector • Increases confidence with unattended automated runs • Single waste point • Ensures capture of all wastes • Easy to connect to waste vessel • Built-in solvent bottle storage • Improved safety and convenience • Lower hight than modular systems • Bottle management software • Protects columns and results on unattended automated runs

  22. Varian 940-LC Integrated HPLC • Special features of the Varian 940-LC • Clear front doors • Stylish • Provides access to autosampler without opening doors • Enhanced safety as lab staff shielded from solvent spray and leaks (100ml/min at 4000 psi) • Useful, convenient storage compartments • Column rack in door • Operation log drawer

  23. Varian 920-LC Integrated HPLC • Special features of the Varian 920-LC • Leak detector • Increases confidence with unattended automated runs • Single waste point • Ensures capture of all wastes • Easy to connect to waste vessel • Built-in Solvent bottle storage • Improved safety and convenience • Lower hight than modular systems

  24. Varian 920-LC Integrated HPLC • Special features of the Varian 920-LC • Clear front doors • Stylish • Provides access to autosampler without opening • Safety increased as lab staff shielded from solvent spray and leaks • Useful, convenient storage compartments • Column rack in door • Operation log drawer

  25. Varian 920-LC AIQ (Validation) • AIQ (Analytical Instrument Qualification) • Fully PC controlled IQ and OQ (not PQ) • Puts the Varian 920-LC at the forefront of regulated capabilities • Consistent, reproducible methodology • Includes pumps, autosampler, detectors, column heater • Chargeable option • Fully documented

  26. 900 LC -Series AIQ • Full system test designed and implemented as part of the 900-LC Series Analytical Instrument Qualification package. • Verifies the entire LC system by systematically running the following • Flow Rate Accuracy Test • Wavelength Detector Accuracy Test • Auto-sampler Injector Accuracy Test (also measures Detector Linearity) • Auto-sampler Injector Precision Test • Auto-sampler Injector Carryover Test • Proportioning Accuracy Test • Detector Noise Test • Detector Drift Test • Auto-sampler Vial Identification Accuracy Test • Proportioning Linearity • Compositional Ripple

  27. Varian 940-LC Configurations -XX where 00=AUS, 01=Eur, 02=US

  28. Varian 940-LC Consumables • Columns designed for Prep users • From 3 to 10mm media • Wide range of C4 to C18 • Silica and Polymer media • From 2mm to 2” ID • Unique solutions for dedicated prep with load and lock systems • Matched Analytical to Prep columns and packing's to assure smooth scale up • Solutions for hardware, software and consumables

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