1 / 32

Bioreactors:

Bioreactors:. Presented by: Shehneela Baseer 117113 Zainab Sajjad 117114. Contents. Introduction to Bioreactors Types of Bioreactor designs Conclusion. Bioreactors. Any manufactured or engineered device or system that supports a biologically active environment.

stricker
Download Presentation

Bioreactors:

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Bioreactors: Presented by: Shehneela Baseer 117113 Zainab Sajjad 117114

  2. Contents • Introduction to Bioreactors • Types of Bioreactor designs • Conclusion

  3. Bioreactors • Any manufactured or engineered device or system that supports a biologically active environment

  4. Types of Bioreactor designs • Stirred tank reactors • Bubble-column reactors • Air lift reactors • Drum rotating reactors • Immobilized plane cell reactors • Membrane reactors

  5. Stirred tank reactors • Air is dispersed by mechanical agitation.

  6. Advantages • Better control over the environment of the culture.

  7. Disadvantages • Can cause damage to the cells • High energy demand • Complexity in construction • Difficult to scale up.

  8. Bubble-column reactors • One of the simplest type of gas – liquid bioreactors.

  9. Advantages • Facilitates sterile operation • Less damaging to shear-sensitive cells • Scale up is relatively easy.

  10. Disadvantages • Undefined fluid flow pattern inside the reactor. • Non-uniform mixing.

  11. Air-lift reactors • Works on draught tube principle.

  12. Advantages • Reasonable mixing with low shear • Operating cost is low. • Less contamination

  13. Disadvantages • Insufficient mixing at high cell densities.

  14. Drum rotating reactors • Consists of horizontally rotating-drum on rollers connected to a motor.

  15. Advantages • High oxygen transfer. • Good mixing • Facilitated better growth and impart less hydrodynamic stress.

  16. Disadvantages • Difficult to scale up.

  17. Immobilized plane cell reactors • Immobilization of plant cell into a suitable carriers. • Either in natural (alginate, agar) or synthetic (polyacrylamide)

  18. Membrane reactors • Cells are separated from growth medium by membrane

  19. Advantages • Environment is more easily controlled • Better control over cell density.

  20. Example: • Insulin Bioreactor Design

  21. Reactor Considerations: • Production of insulin precursor • 5000 kg insulin per year • Assumes 20 % loss due to purification • kLa within 10% of 2088 hr -1 • Prevents oxygen limited reaction • Prevents anaerobic metabolism • Glucose concentration < 0.5 g/L • Prevents formation of ethanol • CSTR configuration • Jacket heat exchanger • Price

  22. How Unit Operates • Substrate feed • Glucose, ammonia, mineral salts • Cellular metabolism of substrate • Extracellular production of insulin • Air sparging for oxygen delivery • Impellers for mixing of nutrients and oxygen

  23. Conclusion • Hence, with the help of different types of bioreactors, commercial production of secondary metabolites is not only possible but also profitable.

  24. Thank you !

More Related