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Reminders

Reminders. Exam 2: Wed, Feb 20: Cytokines Ch 2 & 4 Antibody function- part of chapter 2 will not be on this exam. . WALKER BIOLOGY CLUB MEETING & GUEST SPEAKER!!. Dr. Larry Grand from N.C. State’s Plant Pathology Department will be speaking!

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Reminders

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  1. Reminders • Exam 2: Wed, Feb 20: • Cytokines • Ch 2 & 4 • Antibody function- part of chapter 2 will not be on this exam.

  2. WALKER BIOLOGY CLUB MEETING & GUEST SPEAKER!! Dr. Larry Grand from N.C. State’s Plant Pathology Department will be speaking! Come hear about the Plant Pathology Graduate Program at N.C. State, as well as graduate programs & applications in general! February 27, 2008 5:00 p.m. in Room 210 Pizza & Drinks Provided

  3. B cell Development Chapter 4

  4. B cell tumors provide are used to examine B cell development & provide insight on B cell homing properties. Reed sternberg cells

  5. Bi-lobed nucleus

  6. Probe= J region

  7. Antigen Recognition by T Lymphocytes Chapter 3

  8. Minireview on T cells:

  9. The MHC • MHC structure? • How do antigenic peptides bind into the MHC molecule? • How the the antigenic peptides get processed & presented on the MHC?

  10. Overview of the MHC: • HLA- Human Leukocyte Antigen • Swine: SLA; Bovine: BoLA; Mouse: H-2 • Why is the MHC significant? • Induces a T cell response • Transplant Rejection • Other?

  11. The Structure of the MHC class I & II molecules:

  12. Noncovalent association Ig-Fold MHC class I molecule 45 kDa 12 kDa

  13. Ig Fold MHC class II Molecule 28 kDa 33 kDa Fig. 3.13, 2005

  14. The binding cleft: Fig. 3.15, Parham, 2nd edition

  15. Red= MHC class I Blue= MHC class II Kuby, 4th ed.

  16. Peptide Binding to MHC molecules

  17. MHC class I MHC class II Peptide Binding to MHC molecules

  18. A single MHC can bind to many peptide with each having different AA sequences. MHC molecules have degenerate binding specificity:

  19. Anchor residues: Interact directly with the MHC. Amino terminus Carboxy terminus- hydrophobic/basic residues. Varies with haplotype. 8-10 AA in length (9 preferred). Peptide binding to MHC class I • Middle bulges or kinks

  20. Peptide binding to MHC class I

  21. 13-25 AA “Hot dog on a bun” Peptide in extended conformation. Central 13 AA H bond to MHC cleft. Anchoring not as well defined No bulge. Peptide binding to MHC class II

  22. Anchoring in MHC class II Mouse class II Ak allele HLA-DR3

  23. Antigen Processing & Presentation

  24. Intracellular pathogens with their Ag in the cytosol Ag from Extracellular pathogens is generally internalized by phagocytosis / endocytosis The method of Ag processing can be dependent upon whether the Ag is extracellular or intracellular

  25. Endogenous Pathway Exogenous Pathway

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