10 likes | 152 Views
A. B. Mononuclear leukemia cells from the same patient (45 x 10 6 cells). Mononuclear leukemia cells from the same patient (15 x 10 6 cells). TRIzol RNA isolation (5 x 10 6 cells in each aliquot). Preparation method A. Preparation method A. TRIzol RNA isolation
E N D
A B Mononuclear leukemia cells from the same patient (45 x 106 cells) Mononuclear leukemia cells from the same patient (15 x 106 cells) TRIzol RNA isolation (5 x 106 cells in each aliquot) Preparation method A Preparation method A TRIzol RNA isolation (10 x 106 cells) QIAshredder homogenization (5 x 106 cells) QIAshredder homogenization (5 x 106 cells in each aliquot) Preparation method C RNA Cell lysate RNA Preparation method C Cell lysate RNeasy total RNA purification RNeasy total RNA purification RNeasy total RNA purification RNeasy total RNA purification Preparation method B Preparation method B HG-U133 Plus 2.0 HG-U133 Plus 2.0 HG-U133 Plus 2.0 HG-U133 Plus 2.0 HG-U133 Plus 2.0 HG-U133 Plus 2.0